Vitamin D in individuals before onset of rheumatoid arthritis - relation to vitamin D binding protein and its associated genetic variants
Vitamin D in individuals before onset of rheumatoid arthritis - relation to vitamin D binding protein and its associated genetic variants
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DOI：
10.1186/s41927-018-0033-8
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发表时间：
2018-01-01
期刊：
影响因子：
2.2
通讯作者：
Rantapaa-Dahlqvist, Solbritt
中科院分区：
文献类型：
--
作者：
Brink, Mikael;Johansson, Linda;Rantapaa-Dahlqvist, Solbritt
研究背景维生素D参与了包括类风湿性关节炎（RA）在内的多种自身免疫性疾病的发病机制。之前的研究提出了相互矛盾的结果。维生素D结合蛋白（DBP）是主要的转运蛋白，也参与各种炎症过程。本研究的目的是调查北方瑞典前瞻性队列中症状前个体和匹配对照组的25-羟基维生素D [25（OH）D]、DBP循环水平与组特异性组分（GC）多态性之间的关系。（n=515，症状发作前平均[SD]时间6.2 [9.3]年）和匹配的（2：1）基于人群的对照（n=267）。采用液相色谱串联质谱法分析血浆25（OH）维生素D水平，采用酶联免疫吸附试验分析DBP水平。GC多态性（rs 4588和rs7041）进行了分析与TaqMan检测（应用生物系统公司）。Results 25（OH）D或DBP水平没有统计学差异的前症状的个人和对照组在一个原油，或多重调整的逻辑回归模型。然而，在多重校正模型中，发现DBP每10 mg/L（OR 1.014 [95%CI 1.001-1.028]）校正rs 4588次要等位基因携带后，女性未来患RA的风险增加（p
BackgroundVitamin D has been implicated as being involved in the aetio-pathogenesis of several autoimmune diseases including rheumatoid arthritis (RA). Previous studies present contradictory results. Vitamin D binding protein (DBP), the major transport protein, is also involved in various inflammatory processes. The aim of this study was to investigate the relationship between circulating levels of 25-hydroxyvitamin D [25(OH) D], DBP and polymorphisms in group-specific component (GC) in pre-symptomatic individuals and matched controls within prospective cohorts of the Northern Sweden.MethodsBlood samples donated to the Medical Biobank prior to the onset of symptoms of RA (n=515, mean [SD] time before the onset of symptoms 6.2 [9.3] years) and from matched (2:1) population-based controls (n=267) were used. Plasma 25(OH) vitamin D levels were analyzed using liquid chromatography tandem-mass spectrometry and DBP levels were analyzed using enzyme-linked immunosorbent assay. GC polymorphisms (rs4588 and rs7041) were analyzed with TaqMan assays (Applied Biosystems).ResultsLevels of 25(OH) D or DBP were not statistically different between pre-symptomatic individuals and controls in a crude, or a multiple-adjusted logistic regression model. However, an increased risk for future RA was found in females of DBP (OR 1.014 [95%CI 1.001-1.028]) per 10 mg/L adjusted for carriage of the minor allele of rs4588, in a multiple-adjusted model (p