Antioxidative and anticancer properties of Licochalcone A from licorice
Antioxidative and anticancer properties of Licochalcone A from licorice
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甘草中甘草查耳酮 A 的抗氧化和抗癌特性
DOI：
10.1016/j.jep.2017.01.028
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发表时间：
2017-02-23
影响因子：
5.4
通讯作者：
Guo, Na
中科院分区：
文献类型：
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作者：
Chen, Xiangrong;Liu, Zuojia;Guo, Na
民族药理学相关性：甘草查尔酮A（LCA）是一种特征性的查尔酮，存在于传统药用植物甘草中。LCA在传统医学中具有抗寄生虫、抗炎、抗肿瘤等多种潜在的生物活性，本研究的目的：测定LCA的抗氧化活性，并在此基础上探讨其抗肿瘤活性的作用。为了验证LCA的抗氧化活性，CAT和GPx 1使用蛋白质印迹和细胞抗氧化活性（CAA）测定进行了分析。氧化自由基与癌细胞有关。因此，还评价了LCA的抗癌活性。为了评估抗癌活性，进行细胞活力测定并评价细胞凋亡。结果：实验数据表明，在有或无PBS的两种条件下，LCA的EC_（50）分别为58.79 ± 0.05 μ g/mL和46.29 ± 0.05 μ g/mL。另外，浓度约为2-8 pg/mL的LCA可诱导SOD、CAT和GPx 1蛋白的表达。结论：LCA可抑制HepG 2细胞的氧化损伤，抑制肿瘤细胞的增殖，并可诱导肿瘤细胞凋亡，其作用机制与p38/JNK/ERK信号通路的抑制有关。这些发现证实了LCA在民间医学中的传统用途。
Ethnopharmacological relevance: Licochalcone A (LCA) is a characteristic chalcone that is found in licorice, which is a traditional medicinal plant. In traditional medicine, LCA possesses many potential biological activities, including anti-parasitic, anti-inflammatory and antitumor activities.Aim of the study: To determine the antioxidant activity of LCA and, on this basis, to investigate the role of its anticancer activity.Materials and methods: To validate the antioxidant activity of LCA, the proteins SOD, CAT and GPx1 were analyzed using western blotting and cellular antioxidant activity (CAA) assays. Oxidative free radicals are associated with cancer cells. Therefore, the anticancer activity of LCA was also evaluated. To assess the anticancer activity, cell viability assays were performed and apoptosis was evaluated. In addition, MAPK-related proteins were analyzed using western blotting.Results: The experimental data showed that the EC50 of LCA is 58.79 +/- 0.05 mu g/mL and 46.29 +/- 0.05 mu g/mL under the two conditions tested, with or without PBS. In addition, LCA at a concentration of approximately 2-8 pg/mL can induce the expression of SOD, CAT and GPx1 proteins. Further, LCA inhibits the growth of HepG2 cells through cell proliferation arrest and the subsequent induction of apoptosis, and LCA attenuated the p38/JNK/ERK signaling pathway in a dose-dependent manner.Conclusion: The results showed that LCA suppresses the oxidation of cells and markedly inhibits the proliferation of cancer cells. These findings confirm the traditional use of LCA in folk medicine.