The effect of interleukin-6 on ciliary beat frequency in the human fallopian tube
The effect of interleukin-6 on ciliary beat frequency in the human fallopian tube
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DOI：
10.1016/j.fertnstert.2007.07.1379
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发表时间：
2008-08-01
影响因子：
6.7
通讯作者：
Lyons, R. A.
中科院分区：
文献类型：
--
作者：
Papathanasiou, A.;Djahanbakhch, O.;Lyons, R. A.
目的：探讨白细胞介素-6 （IL-6）对体外人输卵管纤毛活性的影响。设计：以实验室为基础的实验研究。单位：大学教学医院。患者：输卵管标本来自8名因子宫肌瘤而行子宫切除术的妇女。主要观察指标：人输卵管纤毛搏动频率（CBF）。干预(S)：基线CBF测量后，增加IL-6浓度（10pg/mL， 100pg /mL和1000pg /mL）应用于输卵管粘膜外植体，并重复CBF测量。加入抗il -6单克隆抗体，再次测定CBF。使用阴性和仅抗体对照样本。结果：当IL-6浓度为10 pg/mL和100 pg/mL时，CBF保持不变，但IL-6浓度为1000 pg/mL时，CBF显著降低，这种作用被添加特异性抗体所消除。结论：这些数据表明fL-6对纤毛活性有抑制作用，提示该细胞因子可能在低生育过程中起作用。这一发现在腹膜液中IL-6水平升高的情况下可能是重要的，如神经内膜异位症和盆腔炎。
Objective: To investigate the effect of interleukin-6 (IL-6) on ciliary activity within the human fallopian tube in vitro.Design: An experimental I aboratory- based study. Setting: University teaching hospital.Patient(s): Fallopian tube specimens were obtained from eight women undergoing hysterectomy for fibroid uterus.Main Outcome Measure(s): Ciliary beat frequency (CBF) in the human fallopian tube.Intervention(S): Afterbaseline CBF measurements, increasing concentrations of IL-6 (10pg/mL, 100 pg/mL, and 1000 pg/mL) were applied to fallopian tube mucosa explants and CBF measurements repeated. An anti-IL-6 monoclonal antibody was added and CBF measured once more. Negative and antibody-only control samples were used.Result(s): The CBF remained unchanged with the addition of IL-6 at concentrations of 10 pg/mL and 100 pg/mL but IL-6 at 1000 pg/mL significantly decreased CBF, an effect abolished by the addition of the specific antibody.Conclusion(s): These data suggest that fL-6 has an inhibitory effect on ciliary activity, suggesting a possible role for this cytokine in the subfertility process. This finding may be important in conditions where there are increased IL-6 levels in the peritoneal fluid, such as endornetriosis and pelvic inflammatory disease.