Myocardial CXCR4 Expression Is Required for Mesenchymal Stem Cell Mediated Repair Following Acute Myocardial Infarction
Myocardial CXCR4 Expression Is Required for Mesenchymal Stem Cell Mediated Repair Following Acute Myocardial Infarction
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DOI：
10.1161/circulationaha.111.082453
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发表时间：
2012-07-17
期刊：
影响因子：
37.8
通讯作者：
Penn, Marc S.
中科院分区：
文献类型：
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作者：
Dong, Feng;Harvey, James;Penn, Marc S.
基质细胞衍生因子-1在损伤组织中的过度表达导致终末器官功能的改善。在这项研究中，我们量化的间充质干细胞（MSC）基质细胞衍生因子-1释放的局部营养作用的MSC植入在心肌急性myocardial infarction.Methods和Results-Conditional心肌细胞CXCR 4（CM-CXCR 4）null小鼠产生的影响，通过使用他莫昔芬诱导的心脏特异性cre交叉CXCR 4 floxed与MCM-cre小鼠。在急性心肌梗死前3周，在同窝出生的小鼠中进行了（CM-CXCR 4无效）或无（对照）他莫昔芬注射的研究。急性心肌梗死后第1天，小鼠经尾静脉接受100 000 MSC或生理盐水。我们表明α-肌球蛋白重链MerCreMer和MLC-2 v启动子在心脏祖细胞中具有活性。野生型小鼠MSC移植降低了末端脱氧核苷酸转移酶介导的dUTP缺口末端标记阳性CM（-44%，P
Background-Overexpression of stromal cell-derived factor-1 in injured tissue leads to improved end-organ function. In this study, we quantify the local trophic effects of mesenchymal stem cell (MSC) stromal cell-derived factor-1 release on the effects of MSC engraftment in the myocardium after acute myocardial infarction.Methods and Results-Conditional cardiac myocyte CXCR4 (CM-CXCR4) null mice were generated by use of tamoxifen-inducible cardiac-specific cre by crossing CXCR4 floxed with MCM-cre mouse. Studies were performed in littermates with (CM-CXCR4 null) or without (control) tamoxifen injection 3 weeks before acute myocardial infarction. One day after acute myocardial infarction, mice received 100 000 MSC or saline via tail vein. We show alpha-myosin heavy chain MerCreMer and the MLC-2v promoters are active in cardiac progenitor cells. MSC engraftment in wild-type mice decreased terminal deoxynucleotidyltransferase-mediated dUTP nick-end labeling positive CM (-44%, P