Myosin-X is an unconventional myosin that undergoes intrafilopodial motility
Myosin-X is an unconventional myosin that undergoes intrafilopodial motility
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DOI：
10.1038/ncb762
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发表时间：
2002-03-01
影响因子：
21.3
通讯作者：
Cheney, RE
中科院分区：
文献类型：
--
作者：
Berg, JS;Cheney, RE
丝状伪足是细的细胞突起，在细胞运动和神经元生长锥引导中是重要的。构成丝状伪足核心的肌动蛋白丝持续逆行流向细胞体（1，2）。表面受体或颗粒可以耦合到这种逆行流（3，4），也可以向前移动到丝状伪足的尖端（4，5），尽管向前运输的分子基础是未知的。我们在此报道，肌球蛋白X（Myo10或M10）是一类新型肌球蛋白的创始成员（6），定位于丝状伪足的尖端，并在丝状伪足内进行显著的向前和向后运动，我们称之为丝状伪足内运动。GFP-M10斑点的运动对应于相致密颗粒沿着丝状伪足的向前和向后运动。最后，过表达全长M10（但不是M10的截短形式）导致丝状伪足的数量和长度增加，表明M10或其货物可能在丝状伪足动力学中起作用。M10的定位和运动强烈表明，它的功能作为一个电机内filopodial运动。
Filopodia are thin cellular protrusions that are important in cell motility and neuronal growth cone guidance. The actin filaments that make up the core of a filopodium undergo continuous retrograde flow towards the cell body(1,2). Surface receptors or particles can couple to this retrograde flow(3,4) and can also move forward to the tips of filopodia(4,5), although the molecular basis of forward transport is unknown. We report here that myosin-X (Myo10 or M10), the founding member of a novel class of myosins(6), localizes to the tips of filopodia and undergoes striking forward and rearward movements within filopodia, which we term intrafilopodial motility. The movements of the GFP-M10 puncta correspond to forward and rearward movements of phase-dense granules along the filopodia. Finally, overexpressing full-length M10 (but not truncated forms of M10) causes an increase in the number and length of filopodia, indicating that M10 or its cargo may function in filopodial dynamics. The localization and movements of M10 strongly suggest that it functions as a motor for intrafilopodial motility.