The experimental model of transition from compensated cardiac hypertrophy to failure created by transverse aortic constriction in mice.
The experimental model of transition from compensated cardiac hypertrophy to failure created by transverse aortic constriction in mice.
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DOI：
10.1016/j.ijcha.2016.03.007
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发表时间：
2016-06
期刊：
影响因子：
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通讯作者：
Tsutsui H
中科院分区：
文献类型：
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作者：
Furihata T;Kinugawa S;Takada S;Fukushima A;Takahashi M;Homma T;Masaki Y;Tsuda M;Matsumoto J;Mizushima W;Matsushima S;Yokota T;Tsutsui H
横主动脉缩窄（TAC）手术被用作小鼠左心室（LV）肥大和左心室衰竭的实验模型。左心室重塑或衰竭的严重程度可能取决于 TAC 的程度，但在手术动物之间存在差异。因此，我们试图确定 TAC 的最佳直径，以轻松且高再现性地创建该模型。为了在 C57BL/6J 小鼠（7-9 周，体重 19-26 g，n = 109）中产生 TAC，将 7-0 尼龙缝合线紧紧绑在横主动脉周围，并用 3 种不同直径 (mm) 的针固定； 0.40、0.385 和 0.375。通过超声心动图测量左心室壁厚度、舒张末期尺寸、缩短分数。 TAC 后 4 周，没有 0.400 mm 规格的小鼠出现左室衰竭。与 0.375 毫米针规小鼠相比，0.385 毫米针规小鼠的存活率更高（59% 比 48%），这表明在 LV 衰竭方面具有相同的效率。使用 0.385 毫米针规，TAC 后 1 周时，小鼠心脏仍保持左室肥厚，然后在 4 周时出现左室衰竭。直径为 0.385 mm 的 TAC 可以有效诱导小鼠从左心室肥厚向衰竭的转变，且存活率相对较高。
Transverse aortic constriction (TAC) operation is used as an experimental model of left ventricular (LV) hypertrophy and LV failure in mice. The severity of LV remodeling or failure may depend on the degree of TAC, but is variable among operated animals. Therefore, we tried to identify the optimal diameter of TAC to create this model with ease and high reproducibility. To produce TAC in C57BL/6J mice (7–9 weeks, body weight 19–26 g, n = 109), a 7–0 nylon suture ligature was tightly tied around the transverse aorta against needles with 3 different diameters (mm); 0.40, 0.385 and 0.375. LV wall thickness, end-diastolic dimension, fractional shortening were measured by echocardiography. At 4 weeks after TAC, no mouse with the 0.400 mm gauge progressed in LV failure. The 0.385 mm pin gauge mouse kept a more survival rate compared with the 0.375 mm (59% vs 48%), representing same efficient in LV failure. With the 0.385 mm pin gauge, hearts of mice remained LV hypertrophy at 1 week after TAC, followed by LV failure at 4 weeks. TAC with the diameter of 0.385 mm can effectively induce the transition from LV hypertrophy to failure in mice with relatively preserved survival.