Activation of Cryptic Secondary Metabolite Biosynthesis in Bamboo Suspension Cells by a Histone Deacetylase Inhibitor
Activation of Cryptic Secondary Metabolite Biosynthesis in Bamboo Suspension Cells by a Histone Deacetylase Inhibitor
复制标题
组蛋白脱乙酰酶抑制剂激活竹悬浮细胞中隐性次生代谢物生物合成
DOI：
10.1007/s12010-021-03629-2
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发表时间：
2021
影响因子：
3
通讯作者：
Kato Yasuo
中科院分区：
文献类型：
--
作者：
Nomura Taiji;Yoneda Akari;Ogita Shinjiro;Kato Yasuo
植物进化出了多种多样的次生代谢物生物合成途径。然而，未分化的植物细胞倾向于在较小程度上合成次生代谢物，有时根本不合成。培养细胞中的这种现象与表观遗传改变（如低组蛋白乙酰化水平和/或高DNA甲基化水平）导致的生物合成基因转录抑制有关。本文以竹子（Bambusa multiplex; Bm）培养细胞为模型系统，研究了组蛋白去乙酰化酶（histone deacetylase， HDAC）抑制剂对隐次生代谢物生物合成激活的影响。在HDAC抑制剂亚羟基肟酸（suberoylbis- hydroxyamic acid， SBHA）存在下培养的Bm悬浮细胞，表现出对某些在Bm细胞中固有的低水平化合物的强生物合成。分离得到两种主要化合物，分别为3- o -对香豆酰奎宁酸(1)和3- o -阿魏酰奎宁酸(2)。它们的产量与基础培养基类型、初始细胞密度、培养周期以及SBHA浓度有关。另一种HDAC抑制剂trichostatin a也能诱导这两种化合物的生物合成。这些结果表明，HDAC抑制剂可以激活培养植物细胞中隐代代谢物的生物合成。
Plants have evolved a diverse array of secondary metabolite biosynthetic pathways. Undifferentiated plant cells, however, tend to biosynthesize secondary metabolites to a lesser extent and sometimes not at all. This phenomenon in cultured cells is associated with the transcriptional suppression of biosynthetic genes due to epigenetic alterations, such as low histone acetylation levels and/or high DNA methylation levels. Here, using cultured cells of bamboo (Bambusa multiplex; Bm) as a model system, we investigated the effect of histone deacetylase (HDAC) inhibitors on the activation of cryptic secondary metabolite biosynthesis. The Bm suspension cells cultured in the presence of an HDAC inhibitor, suberoylbis-hydroxamic acid (SBHA), exhibited strong biosynthesis of some compounds that are inherently present at very low levels in Bm cells. Two major compounds induced by SBHA were isolated and were identified as 3-O-p-coumaroylquinic acid (1) and 3-O-feruloylquinic acid (2). Their productivities depended on the type of basal culture medium, initial cell density, and culture period, as well as the SBHA concentration. The biosynthesis of these two compounds was also induced by another HDAC inhibitor, trichostatin A. These results demonstrate the usefulness of HDAC inhibitors to activate cryptic secondary metabolite biosynthesis in cultured plant cells.