Enhancing the performance of the light field microscope using wavefront coding
Enhancing the performance of the light field microscope using wavefront coding
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DOI：
10.1364/oe.22.024817
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发表时间：
2014-10-06
期刊：
影响因子：
3.8
通讯作者：
Levoy, Marc
中科院分区：
文献类型：
--
作者：
Cohen, Noy;Yang, Samuel;Levoy, Marc
光场显微镜已被提出作为一种新的高速体积计算成像方法，使重建的3-D体积从捕获的投影的4-D光场。最近，一个详细的物理光学模型的光场显微镜已经推导出，这导致了一个反卷积算法，重建3-D体积具有高空间分辨率的发展。然而，重建的空间分辨率已被证明是跨深度不均匀的，一些z平面显示高分辨率，而其他的，特别是在成像体积的中心，显示非常低的分辨率。在本文中，我们使用波前编码技术来提高光场显微镜的性能。通过在显微镜的光路中包括相位掩模，我们能够解决这种不均匀的分辨率限制。我们还发现，上级控制的光场显微镜的性能可以通过使用两个相位掩模，而不是一个，放置在物镜的后焦平面和显微镜的原生图像平面。我们提出了一个扩展的光学模型，我们的波前编码光场显微镜，并开发了一个性能指标的基础上费舍尔信息，我们使用它来选择适当的相位掩模参数。我们验证我们的方法，使用模拟数据和实验分辨率测量的USAF 1951年的分辨率目标，并证明了实用程序的生物学应用与体内体积钙成像的幼斑马鱼大脑。(C)2014美国光学学会
Light field microscopy has been proposed as a new high-speed volumetric computational imaging method that enables reconstruction of 3-D volumes from captured projections of the 4-D light field. Recently, a detailed physical optics model of the light field microscope has been derived, which led to the development of a deconvolution algorithm that reconstructs 3-D volumes with high spatial resolution. However, the spatial resolution of the reconstructions has been shown to be non-uniform across depth, with some z planes showing high resolution and others, particularly at the center of the imaged volume, showing very low resolution. In this paper, we enhance the performance of the light field microscope using wavefront coding techniques. By including phase masks in the optical path of the microscope we are able to address this non-uniform resolution limitation. We have also found that superior control over the performance of the light field microscope can be achieved by using two phase masks rather than one, placed at the objective's back focal plane and at the microscope's native image plane. We present an extended optical model for our wavefront coded light field microscope and develop a performance metric based on Fisher information, which we use to choose adequate phase masks parameters. We validate our approach using both simulated data and experimental resolution measurements of a USAF 1951 resolution target; and demonstrate the utility for biological applications with in vivo volumetric calcium imaging of larval zebrafish brain. (C) 2014 Optical Society of America