Analytical equilibrium gradient methods
Analytical equilibrium gradient methods
复制标题
分析平衡梯度法
DOI：
10.1007/s00216-002-1286-y
复制
发表时间：
2002
影响因子：
4.3
通讯作者：
Milton L. Lee
中科院分区：
文献类型：
--
作者：
Qinggang Wang;Dennis H. Tolley;D. Lefebre;Milton L. Lee
解析平衡梯度法是一种非线性分离方法，其分离机理涉及沿分离通道沿着的力梯度。这些方法可以分为两类：其中梯度是沿分离通道沿着施加的场梯度（即，场梯度），以及其中通道经受具有以某种其它性质形成的梯度的恒定场的那些（即，常数域）。峰宽标准偏差、分离度和峰容量是表征平衡梯度法的重要参数，一般表达式可从作用点和基本通量方程两方面考虑。介绍了密度梯度沉降、等电聚焦、电迁移率聚焦等几个成功的应用实例。基于场梯度类的平衡梯度法，提出了一种动态提高峰值容量的方法。这种方法的一个例子是使用电迁移率聚焦。
Abstract Analytical equilibrium gradient methods are non-linear separation methods in which the separation mechanism involves a force gradient along the separation channel. These methods can be classified into two categories: those in which the gradient is a field gradient applied along the separation channel (i.e., field gradient), and those in which the channel is subjected to a constant field with a gradient formed in some other property (i.e., constant field). Standard deviation of peak width, resolution and peak capacity are important parameters in characterizing equilibrium gradient methods, and general expressions can be obtained from considering both the point of force acting on the analyte and the basic flux equation. Several successful examples, such as density gradient sedimentation, isoelectric focusing and electromobility focusing are discussed. Based on equilibrium gradient methods in the field gradient category, a method to dynamically improve peak capacity is described. An example of such an approach is given using electromobility focusing.