Involvement of specific integrins in apoptosis induced by vascular apoptosis-inducing protein 1
Involvement of specific integrins in apoptosis induced by vascular apoptosis-inducing protein 1
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DOI：
10.1016/s0041-0101(01)00249-5
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发表时间：
2002-05-01
期刊：
影响因子：
2.8
通讯作者：
Hayashi, H
中科院分区：
文献类型：
--
作者：
Araki, S;Masuda, S;Hayashi, H
出血性蛇毒诱导血管内皮细胞凋亡。在以前的报告中，我们描述了纯化和cDNA克隆从响尾蛇血管凋亡诱导蛋白1（VAP 1），特异性诱导血管内皮细胞凋亡。VAP 1属于金属蛋白酶/去整合素家族。然而，VAP 1诱导细胞凋亡的机制仍不清楚。由于已知蛇毒中的其他各种金属蛋白酶和去整合素影响细胞外基质和细胞粘附，我们在此研究这些粘附分子在VAP 1诱导的凋亡中的参与。因此，VAP 1诱导的凋亡不降解细胞外基质或抑制VEC的粘附。然而，VAP 1诱导的细胞凋亡被整合素α 3、α 6、β 1的抗体抑制。此外，细胞凋亡被CD 9（一种整合素相关蛋白）抗体抑制。这些结果表明，整合素参与VAP 1诱导的细胞凋亡的一些特定的作用，而不是粘附到细胞外基质。(C)2002爱思唯尔科技有限公司。保留所有权利。
Hemorrhagic snake venom induces apoptosis in vascular endothelial cells (VEC). In previous reports, we described the purification and cDNA cloning from Crotalus atrox of vascular apoptosis-inducing protein 1 (VAP1) that specifically induces apoptosis in VEC. VAP1 belongs to the metalloprotease/disintegrin family. Yet the mechanism of inducing apoptosis by VAP1 is still not known. Since other various metalloproteases and disintegrins in snake venoms are known to influence extracellular matrix and cell adhesion, we investigated here the involvement of these adhesion molecules in VAP1-induced apoptosis, Consequently, VAP1 induced apoptosis without degrading extracellular matrix or inhibiting adhesion of VEC. However, VAP1-induced apoptosis was inhibited by antibodies for integrin alpha3, alpha6, beta1. Additionally, apoptosis was inhibited by antibody for CD9, an integrin associated protein. These results suggest that integrins are involved in VAP1-induced apoptosis by some specific role rather than that of adhesion to extracellular matrix. (C) 2002 Elsevier Science Ltd. All rights reserved.