Regulation of the Cln3-Cdc28 kinase by cAMP in Saccharomyces cerevisiae
Regulation of the Cln3-Cdc28 kinase by cAMP in Saccharomyces cerevisiae
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DOI：
10.1093/emboj/17.15.4370
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发表时间：
1998-08-03
期刊：
影响因子：
11.4
通讯作者：
Heideman, W
中科院分区：
文献类型：
--
作者：
Hall, DD;Markwardt, DD;Heideman, W
酿酒酵母在不同的生长介质中的生长速度差异很大。为了保持相对恒定的细胞大小，酵母细胞必须调节细胞周期的进展速率以匹配生长速率的变化，在丰富的培养基中快速通过G(1)，而在贫乏的培养基中缓慢地通过G(1)。我们研究了营养物质与 Cln3-Cdc28 激酶的表达和活性之间的联系，Cln3-Cdc28 激酶调节酵母细胞周期的 G(1)-S 边界，这一点称为“起始点”。我们发现 Cln3 蛋白水平在葡萄糖中最高，在较差的碳源中较低。这种调节涉及转录和转录后控制，尽管 Ras-cAMP 途径似乎不影响 CLN3 转录，但 cAMP 会增加 Cln3 蛋白水平和 Cln3-Cdc28 激酶活性。这种调节需要 CLN3 信息的非翻译区域，并且可以通过 cAMP 引起的蛋白质合成速率的变化来解释。提出了 CLN3 调节和功能的模型，其中 CLN3 响应营养物质调节 G(1) 长度。
The yeast Saccharomyces cerevisiae grows at widely varying rates in different growth media. In order to maintain a relatively constant cell size, yeast cells must regulate the rate of progress through the cell cycle to match changes in growth rate, moving quickly through G(1) in rich medium, and slowly in poor medium. We have examined connections between nutrients, and the expression and activity of Cln3-Cdc28 kinase that regulates the G(1)-S boundary of the cell cycle in yeast, a point referred to as Start. We find that Cln3 protein levels are highest in glucose and lower in poorer carbon sources. This regulation involves both transcriptional and post-transcriptional control, Although the Ras-cAMP pathway does not appear to affect CLN3 transcription, cAMP increases Cln3 protein levels and Cln3-Cdc28 kinase activity. This regulation requires untranslated regions of the CLN3 message, and can be explained by changes in protein synthesis rates caused by cAMP, A model for CLN3 regulation and function is presented in which CLN3 regulates G(1) length in response to nutrients.