Production of porous hydroxyapatite by the gel-casting of foams and cytotoxic evaluation.
Production of porous hydroxyapatite by the gel-casting of foams and cytotoxic evaluation.
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DOI：
10.1002/(sici)1097-4636(200004)50:1
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发表时间：
2000-04
期刊：
影响因子：
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通讯作者：
P. Sepulveda;Jon Binner;S. Rogero;O. Higa;J. C. Bressiani
中科院分区：
文献类型：
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作者：
P. Sepulveda;Jon Binner;S. Rogero;O. Higa;J. C. Bressiani
本研究提出了一种新的技术，采用悬浮液的发泡之前，在原位聚合的组合物中包含的有机单体的高度多孔的羟基磷灰石的制造。该方法可生产孔隙率高达90%的坚固胶凝体，其可承受绿色状态下的机加工。如果该方法包括在一个处理步骤中铸造，则可以获得复杂形状的部件。在高于300摄氏度的温度下消除有机添加剂，并进行烧结以固结陶瓷基质。根据试样密度，尺寸范围为20至1000微米的球形互连单元表征多孔结构。基于多孔材料与哺乳动物细胞间接接触后细胞集落形成和细胞死亡测定的定量方法，对烧结HA泡沫浸提液进行细胞毒性试验。生物学评价的体外试验表明，生物医学级羟基磷灰石粉末的原始纯度既不受加工影响，也不受所用试剂的影响。
This study presents the manufacture of highly porous hydroxyapatite by a novel technique that employs the foaming of suspensions prior to the in situ polymerization of organic monomers contained in the compositions. This method produces strong gelled bodies with up to 90% porosity that can withstand machining in the green state. Complex-shaped components can be obtained if the process comprises casting in one of the processing steps. The organic additives are eliminated at temperatures above 300 degrees C, and sintering is carried out for consolidation of the ceramic matrix. Spherical interconnected cells with sizes ranging from 20 to 1000 micrometer characterize the porous structure, depending on the specimen density. Cytotoxicity tests were conducted on extracts from sintered HA foams based on a quantitative method of cell colony formation and the determination of cell death after indirect contact of the porous material with mammalian cells. This in vitro test of biological evaluation revealed that the original purity of the biomedical-grade hydroxyapatite powder was affected neither through processing nor by the employed reagents.