Age-related changes in human sperm DNA integrity
Age-related changes in human sperm DNA integrity
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DOI：
10.18632/aging.102120
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发表时间：
2019-08-15
期刊：
影响因子：
5.2
通讯作者：
Piasecka, Malgorzata
中科院分区：
文献类型：
--
作者：
Rosiak-Gill, Aleksandra;Gill, Kamil;Piasecka, Malgorzata
随着男性年龄的增长，可能会出现标准精液特征异常和精子染色质成熟度降低。然而，父亲的年龄对精液参数的影响仍然存在争议。因此，本研究旨在评估父亲年龄不仅对常规精液特征的影响，而且对精子DNA完整性的影响。这项研究是在年龄分别为40岁和40岁的男性(n=1124)身上进行的。我们的数据显示，与整个研究队列中的年轻男性、精子正常精子症和精液参数异常的男性相比，老年男性的精液量减少，精子DNA碎片指数(DFI)增加。此外，在40岁年龄组中，精子DNA损伤(10%DFI，低生育潜力)的发生率高于40岁男性组。老年男性高精子DNA损伤的几率是年轻男性的两倍多。我们的发现表明，父亲的高龄对精子染色质的完整性有不利影响。数据表明，在男性生育潜力评估中，精子DNA的评估比标准精液分析具有更大的临床实用价值。
Abnormal standard semen characteristics and reduced sperm chromatin maturity can appear with increasing male age. However, the influence of paternal age on semen parameters is still controversial. Therefore, this study was designed to estimate the influence of paternal age not only on conventional semen characteristics but also on sperm DNA integrity. This research was carried out on ejaculated sperm cells obtained from men (n = 1124) aged >= 40 y and < 40 y. Our data revealed a decreased semen volume and an increased percentage of DFI (sperm DNA fragmentation index) in older men compared to younger men in the entire study cohort, in men with normo-zoospermia and in men with abnormal semen parameters. Moreover, there was a higher incidence of sperm DNA damage (>10% DFI, low fertility potential) in the groups of men aged >= 40 y than in the groups of men aged < 40 y. Older men had over twice the odds ratio for high sperm DNA damage as younger men. Our findings suggest a detrimental effect of advanced paternal age on sperm chromatin integrity. The data show that the evaluation of sperm DNA has greater clinical utility than standard semen analysis in case of male fertility potential assessment.