TEMPORAL AND SPATIAL EXPRESSION PATTERNS OF THE SMALL HEAT-SHOCK (HSP-16) GENES IN TRANSGENIC CAENORHABDITIS-ELEGANS
TEMPORAL AND SPATIAL EXPRESSION PATTERNS OF THE SMALL HEAT-SHOCK (HSP-16) GENES IN TRANSGENIC CAENORHABDITIS-ELEGANS
复制标题
DOI：
10.1091/mbc.3.2.221
复制
发表时间：
1992-02-01
影响因子：
3.3
通讯作者：
CANDIDO, EPM
中科院分区：
文献类型：
--
作者：
STRINGHAM, EG;DIXON, DK;CANDIDO, EPM
通过转化将 hsp16-lacZ 融合体引入线虫中，检查了 hsp16 基因家族在秀丽隐杆线虫中的表达。 hsp16-lacZ 转基因的转录完全依赖于热休克，并导致快速合成可检测水平的 β-半乳糖苷酶。尽管线虫的两个 hsp16 基因对在编码和非编码序列方面高度相似，但观察到基因对之间表达空间模式的定量和定性差异。 hsp16-48 启动子被证明可以指导肌肉和皮下组织中 β-半乳糖苷酶的更大表达，而 hsp16-41 启动子在肠和咽组织中更有效。从基因对中消除一个启动子的转基因表达水平降低，特别是在胚胎后阶段，这表明hsp16基因对基因间区域的热休克元件可能协同作用以实现两个基因的高水平表达。尽管 hsp16 基因对从未组成型表达，但它们的热诱导能力在发育上受到限制；它们在配子发生或早期胚胎发生期间不是热诱导的。 hspl6基因代表了秀丽隐杆线虫中第一个在分子水平上进行详细表征的完全诱导系统，并且这些基因的启动子应该在该实验生物体的组织和发育调节基因的研究中具有广泛的适用性。
The expression of the hsp16 gene family in Caenorhabditis elegans has been examined by introducing hsp16-lacZ fusions into the nematode by transformation. Transcription of the hsp16-lacZ transgenes was totally heat-shock dependent and resulted in the rapid synthesis of detectable levels of beta-galactosidase. Although the two hsp16 gene pairs of C. elegans are highly similar within both their coding and noncoding sequences, quantitative and qualitative differences in the spatial pattern of expression between gene pairs were observed. The hsp16-48 promoter was shown to direct greater expression of beta-galactosidase in muscle and hypodermis, whereas the hsp16-41 promoter was more efficient in intestine and pharyngeal tissue. Transgenes that eliminated one promoter from a gene pair were expressed at reduced levels, particularly in postembryonic stages, suggesting that the heat shock elements in the intergenic region of an hsp16 gene pair may act cooperatively to achieve high levels of expression of both genes. Although the hsp16 gene pairs are never constitutively expressed, their heat inducibility is developmentally restricted; they are not heat inducible during gametogenesis or early embryogenesis. The hspl6 genes represent the first fully inducible system in C. elegans to be characterized in detail at the molecular level, and the promoters of these genes should find wide applicability in studies of tissue- and developmentally regulated genes in this experimental organism.