The fibrinolysis inhibitor α2-antiplasmin in the human cornea
The fibrinolysis inhibitor α2-antiplasmin in the human cornea
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DOI：
10.1080/02713680500403046
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发表时间：
2005-12-01
影响因子：
2
通讯作者：
Yue, BYJT
中科院分区：
文献类型：
--
作者：
Ayala, A;Warejecka, DJ;Yue, BYJT
目的：确定角膜是否在基因水平上含有并表达主要纤溶酶抑制剂α(2)-抗纤溶酶。方法：对角膜切片进行α(2)-抗纤溶酶免疫染色。对人角膜基质、上皮和内皮的提取物进行免疫点印迹和蛋白质印迹分析。总RNA和α(2)-抗纤溶酶特异性引物用于RT-PCR。对cDNA进行测序。结果：通过免疫定位和蛋白质印迹在所有三个角膜层中观察到α(2)-抗纤溶酶。在大多数提取物中观察到的主要 α(2)-抗纤溶酶形式是 70-kDa 形式。总 α(2)-抗纤溶酶的含量为 0.119 +/- 0.014 μg/上皮 (n = 10) 和 1.45 +/- 0.47 μg/基质 (n = 10)。在上皮和基质提取物以及培养的人角膜基质细胞中检测到 α(2)-抗纤溶酶 mRNA。 PCR 产物的序列与人α(2)-抗纤溶酶的序列相同。结论：α(2)-抗纤溶酶及其 mRNA 存在于角膜中，可能有助于调节角膜纤溶酶活性。
Purpose : To determine whether the cornea contains and expresses, at the gene level, the major plasmin inhibitor alpha(2)-antiplasmin. Methods : Corneal sections were immunostained for alpha(2)-antiplasmin. Extracts of human corneal stroma, epithelium, and endothelium were subjected to immunodot blot and Western blot analysis. Total RNA and alpha(2)-antiplasmin specific primers were used for RT-PCR. The cDNA was sequenced. Results : alpha(2)-Antiplasmin was observed in all three corneal layers by immunolocalization and Western blots. The major alpha(2)-antiplasmin form observed in most extracts was the 70-kDa form. Total alpha(2)-antiplasmin was present at 0.119 +/- 0.014 mu g/epithelium (n = 10) and 1.45 +/- 0.47 mu g/stroma (n = 10). alpha(2)-Antiplasmin mRNA was detected in epithelial and stromal extracts and cultured human corneal stromal cells. The sequences of the PCR products were identical to that for human alpha(2)-antiplasmin. Conclusions : alpha(2)-Antiplasmin and its mRNA are present in the cornea and may serve to regulate corneal plasmin activity.