Sequence of the human immunoglobulin diversity (D) segment locus: A systematic analysis provides no evidence for the use of DIR segments, inverted D segments, ''minor'' D segments or D-D recombination
Sequence of the human immunoglobulin diversity (D) segment locus: A systematic analysis provides no evidence for the use of DIR segments, inverted D segments, ''minor'' D segments or D-D recombination
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DOI：
10.1006/jmbi.1997.1141
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发表时间：
1997-07-25
影响因子：
5.6
通讯作者：
Winter, G
中科院分区：
文献类型：
--
作者：
Corbett, SJ;Tomlinson, IM;Winter, G
我们测定了染色体14q32.3上人免疫球蛋白D片段位点的完整核苷酸序列，共鉴定出27个D片段，其中9个是新的。与重排重链序列数据库的比较表明，人抗体库是通过涉及这 27 个 D 片段中的 25 个的 VDJ 重组、V-D 和 D-J 连接处的广泛处理以及多个阅读框的使用而创建的。我们找不到任何证据证明建议使用 DIR 片段、倒置 D 片段、“次要”D 片段或 D-D 重组。因此，遵守 12/23 规则的传统 VDJ 重组足以解释人类重链第三个高变环的丰富长度和序列。 (C) 1997 学术出版社有限公司。
We have determined the complete nucleotide sequence of the human immunoglobulin D segment locus on chromosome 14q32.3 and identified a total of 27 D segments, of which nine are new. Comparison with a database of rearranged heavy chain sequences indicates that the human antibody repertoire is created by VDJ recombination involving 25 of these 27 D segments, extensive processing at the V-D and D-J junctions and use of multiple reading frames. We could find no evidence for the proposed use of DIR segments, inverted D segments, ''minor'' D segments or D-D recombination. Conventional VDJ recombination, which obeys the 12/23 rule, is therefore sufficient to explain the wealth of lengths and sequences for the third hypervariable loop of human heavy chains. (C) 1997 Academic Press Limited.