CELLULAR SPECIFICITY OF SEROTONIN STORAGE AND AXONAL-TRANSPORT IN IDENTIFIED NEURONS OF APLYSIA-CALIFORNICA
CELLULAR SPECIFICITY OF SEROTONIN STORAGE AND AXONAL-TRANSPORT IN IDENTIFIED NEURONS OF APLYSIA-CALIFORNICA
复制标题
DOI：
10.1113/jphysiol.1974.sp010694
复制
发表时间：
1974-01-01
影响因子：
5.5
通讯作者：
SCHWARTZ, JH
中科院分区：
文献类型：
--
作者：
GOLDMAN, JE;SCHWARTZ, JH
1.[~3H]D，L-5-羟色氨酸([~H]~5HTP)加压注射入海贝类中枢神经系统已鉴定的胆碱能和5-羟色胺能神经元胞体。5-羟色胺能神经元和胆碱能神经元均将[~3H]~5HTP转化为[~3H]~5-羟色胺([~3H]~5HT)。[~3H]5-羟色胺在两种类型神经元中的命运不同。在5-羟色胺能细胞中，5-羟色胺主要以颗粒形式存在；递质很容易通过选择性运输从细胞体进入神经。相反，5-羟色胺仍然游离在胆碱能神经元的胞浆中，不能从细胞体转运出去。利血平治疗后，海绵体内颗粒物质结合的[~3H]5-羟色胺比例降低，恢复的[~3H]5-羟色胺含量减少。5-羟色胺能神经元具有胆碱能细胞所不具备的5-羟色胺储存和轴突运输的特殊机制。
1. [3H]D,L‐5‐hydroxytryptophan ([3H]5HTP) was injected under pressure into cell bodies of identified cholinergic and serotonergic neurones in the central nervous system of the marine mollusc,Aplysia californica.2. Both serotonergic and cholinergic neurones converted [3H]5HTP to [3H]5‐hydroxytryptamine ([3H]5HT).3. The fate of [3H]5HT in the two types of neurones differed. In serotonergic cells, 5HT was present primarily in particulate form; the transmitter readily moved from cell bodies into nerves by selective transport. In contrast, 5HT remained free in the cytoplasm of the cholinergic neurone, and was not transported from the cell body.4. Treatment ofAplysiawith reserpine decreased the proportion of [3H]5HT associated with particulate material, and also decreased the amount of [3H]5HT recovered.5. Serotonergic neurones possess specific mechanisms for the storage and axonal transport of 5HT which are absent in cholinergic cells.