Comparative proteomic study between human normal motility sperm and idiopathic asthenozoospermia
Comparative proteomic study between human normal motility sperm and idiopathic asthenozoospermia
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人类正常活力精子与特发性弱精子症的蛋白质组学比较研究
DOI：
10.1007/s00345-013-1023-5
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发表时间：
2013-12-01
影响因子：
3.4
通讯作者：
He, Dalin
中科院分区：
文献类型：
--
作者：
Shen, Shulin;Wang, Jinzi;He, Dalin
目的特发性弱精子症被认为是男性不育的原因之一，其特点是精子活力降低。为了更好地明确弱精子症的发病机制，本研究对正常精子活力和特发性弱精子症中的差异表达蛋白进行了探索。方法采用二维电泳法提取分离精子蛋白。从 2D 凝胶中拾取所有显着变化的蛋白质点，并通过串联质谱法进行鉴定。通过基质辅助激光解吸/电离飞行时间串联质谱法成功鉴定了 33 个差异表达蛋白点中的 16 个。结果鉴定出 16 个蛋白质，属于 15 个独特的蛋白质组。 GRP78、乳铁蛋白、SPANXB、PGK2、鞭毛蛋白、DJ-1、XPA结合蛋白2、CAB2、GPX4和GAPDH是第一个被确定为特发性弱精子症患者差异表达蛋白的蛋白。同时进行定量RT-PCR分析比较蛋白质水平，结果表明基因和蛋白质的表达水平并不完全一致。结论这些实验结果扩大了蛋白质数据库的范围，为进一步研究特发性弱精子症的发病机制提供了靶点。
PurposeIdiopathic asthenozoospermia is considered as one of the causes of male infertility and characterized by reduced sperm motility. For a better determination of pathogenic mechanism of asthenozoospermia, the exploration of differentially expressed proteins in normal sperm motility and idiopathic asthenozoospermia was conducted in our study.MethodsSperm proteins were extracted and isolated by two-dimensional electrophoresis. All significantly changed protein spots were picked up from 2D gels and identified by tandem mass spectrometry. Sixteen of the thirty-three total differentially expressed protein spots were successfully identified by matrix-assisted laser desorption/ionization time-of-flight tandem mass spectrometry.ResultsSixteen proteins identified belonged to 15 unique protein groups. GRP78, lactoferrin, SPANXB, PGK2, flagellin, DJ-1, XPA binding protein 2, CAB2, GPX4, and GAPDH were the first to be identified as differentially expressed proteins in idiopathic asthenospermia patients. Meanwhile, the analysis of quantitative RT-PCR was carried out to compare the protein levels, and the results indicated that the expression levels of the gene and protein were not entirely consistent.ConclusionsThese experimental results expand the scope of the protein database, generating targets for further investigation of the pathogenic mechanism of idiopathic asthenozoospermia.