A TaqMan Probe-Based Real-Time PCR Assay for the Rapid Identification of the Emerging Multidrug-Resistant Pathogen Candida auris on the BD Max System
A TaqMan Probe-Based Real-Time PCR Assay for the Rapid Identification of the Emerging Multidrug-Resistant Pathogen Candida auris on the BD Max System
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DOI：
10.1128/jcm.01604-18
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发表时间：
2019-07-01
影响因子：
9.4
通讯作者：
Silbert, Suzane
中科院分区：
文献类型：
--
作者：
Lima, Amorce;Widen, Raymond;Silbert, Suzane
耳念珠菌是一种新兴的多重耐药真菌病原体，其与医院内血流和深部伤口感染相关，主要在重症监护病房（ICU）患者中导致高死亡率。实验室目前依赖于使用商业自动化系统的表型检测来鉴定酵母菌;然而，这种技术经常导致C.与其他近缘物种的关系。我们在BD Max平台上开发并验证了一种基于TaqMan的实时PCR检测方法，靶向核糖体DNA（rDNA）区域核苷酸序列，以快速准确地检测C.培养和临床标本的耳感染。该检测方法具有高度特异性、重现性和灵敏度，可检测低至1 ℃的样品。在3 h内每个反应产生耳CFU。
Candida auris is an emerging multidrug-resistant fungal pathogen that has been associated with nosocomial bloodstream and deep wound infections causing a high mortality rate mainly in intensive care unit (ICU) patients. Laboratories currently rely on phenotypic testing using commercial automated systems for identification of yeasts; however, this technique has often led to misidentification of C. auris to other closely related species. We developed and validated a TaqMan-based real-time PCR assay on the BD Max platform targeting ribosomal DNA (rDNA) region nucleotide sequences to quickly and accurately test for C. auris infection from culture and clinical specimens. The assay is highly specific, reproducible, and sensitive, allowing detection of as low as 1 C. auris CFU per reaction within 3 h.