Distinction between Nuclear Satellite DNAs and Chloroplast DNA in Higher Plants.

Distinction between Nuclear Satellite DNAs and Chloroplast DNA in Higher Plants.
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高等植物中核卫星 DNA 和叶绿体 DNA 的区别。

DOI:
10.1104/pp.62.6.975
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发表时间:
1978
期刊:
影响因子:
7.4
通讯作者:
John Ingle
John Ingle
中科院分区:
生物学1区
文献类型:
--
作者:
M. J. Pascoe;John Ingle

文献摘要

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Triton X-100溶解了来自叶绿体和细胞核混合物的叶绿体DNA,但不溶解细胞核DNA。不同植物(菜豆、黄瓜、梅洛、金鱼草、蚕豆、月见草)叶绿体DNA和卫星DNA的浮力密度不同。叶绿体DNA占叶片总DNA的4.3%~ 0.25%,每个叶绿体中有5 ~ 20个拷贝。
Triton X-100 solubilized chloroplast DNA but not nuclear DNA from a mixture of chloroplasts and nuclei. The buoyant density of chloroplast DNA was different from that of the satellite DNA in all of the species examined (Phaseolus coccineus, Cucumis sativus, Cucumis melo, Antirrhinum majus, Vicia faba, Oenothera fruiticosa youngii). Chloroplast DNA constituted between 4.3% and 0.25% of the total leaf DNA in these species, and was present as 5 to 20 copies in each chloroplast.