Multiple roles of Arf1 GTPase in the yeast exocytic and endocytic pathways.
Multiple roles of Arf1 GTPase in the yeast exocytic and endocytic pathways.
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Arf1 GTPase 在酵母胞吐和内吞途径中的多重作用。
DOI：
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发表时间：
2001
影响因子：
3.3
通讯作者：
Akihiko Nakano
中科院分区：
文献类型：
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作者：
Natsuko Yahara;Takashi Ueda;Ken Sato;Akihiko Nakano
adp核糖基化因子，一个小的gtpase家族，被认为是细胞膜内运输的关键调节剂。然而，许多体外生化实验得出了它们参与囊泡运输各个步骤的不同模型，它们在活细胞中的确切作用仍不清楚。我们利用强大的酵母遗传系统，筛选了酿酒酵母ARF1基因的温度敏感（ts）突变体。采用易出错PCR对ARF1的整个开放阅读框进行随机诱变，分离出8个突变体，并对其表型进行了检测。Arf1突变体以等位基因特异性的方式表现出多种运输缺陷和形态改变。此外，在某些突变等位基因对之间观察到基因内互补，用于细胞生长和细胞内运输。这些结果表明，单个Arf1蛋白确实参与了体内细胞内运输的许多不同步骤，并且我们构建的突变等位基因可能剖析了它的多种作用。
ADP-ribosylation factors, a family of small GTPases, are believed to be key regulators of intracellular membrane traffic. However, many biochemical in vitro experiments have led to different models for their involvement in various steps of vesicular transport, and their precise role in living cells is still unclear. We have taken advantage of the powerful yeast genetic system and screened for temperature-sensitive (ts) mutants of the ARF1 gene from Saccharomyces cerevisiae. By random mutagenesis of the whole open reading frame of ARF1 by error-prone PCR, we isolated eight mutants and examined their phenotypes. arf1 ts mutants showed a variety of transport defects and morphological alterations in an allele-specific manner. Furthermore, intragenic complementation was observed between certain pairs of mutant alleles, both for cell growth and intracellular transport. These results demonstrate that the single Arf1 protein is indeed involved in many different steps of intracellular transport in vivo and that its multiple roles may be dissected by the mutant alleles we constructed.
arf1Delta 合成致死筛选鉴定出一种新的网格蛋白重链条件等位基因，该等位基因扰乱酿酒酵母中的液泡蛋白运输。
影响因子：
3.3
作者：
Chen,CY;Graham,TR
通讯作者：
Graham,TR
酵母中 Ypt GTPases 和 Arf 鸟嘌呤核苷酸交换器之间的遗传相互作用。
影响因子：
3.3
作者：
Jones,S;Jedd,G;Kahn,RA;Franzusoff,A;Bartolini,F;Segev,N
通讯作者：
Segev,N
DOI：
10.1101/gr.2.1.28
发表时间：
1992-08-01
期刊：
PCR methods and applications
影响因子：
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作者：
Cadwell, R C;Joyce, G F
通讯作者：
Joyce, G F