Human herpesvirus 6 in oral fluids from healthy individuals
Human herpesvirus 6 in oral fluids from healthy individuals
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DOI：
10.1016/j.archoralbio.2004.06.002
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发表时间：
2004-12-01
影响因子：
3
通讯作者：
Barjas-Castro, ML
中科院分区：
文献类型：
--
作者：
Pereira, CM;Gasparetto, PF;Barjas-Castro, ML
背景：人类疱疹病毒6型(HHV6)是引起皮疹的病原体。该病毒潜伏在唾液腺中，唾液是病毒传播的主要形式。这项研究的目的是使用一种标准化的技术从牙龈收集和提取病毒DNA来评估健康个体体液中HHV6的发病率。阴沟液、全唾液和腮腺唾液。设计：口腔液和外周血样。采集28名献血员的血液，用聚合酶链式反应(PCR)方法检测HHV6。用牙髓纸锥收集腮腺唾液和牙周沟液，以避免全唾液污染。结果：在28名献血者中，20名(71.4%)在所研究的三种口服液中至少有一种呈现阳性结果。19例(67.8%)受试者全唾液阳性，仅4例(14.2%)龈沟液和4例腮腺唾液阳性。结论：结果表明，在大部分健康成年人的唾液中存在HHV6。使用牙髓纸锥收集口腔液和提取病毒具有高效、简单、廉价和无痛苦的特点。尽管研究的病例数量很少，但有可能证明，在整个唾液中，牙周沟液和腮腺唾液都不是HHV6的主要来源。(C)2004爱思唯尔有限公司。保留所有权利。
Background: Human herpesvirus 6 (HHV6) is the etiologic agent of exanthem subitum. The virus is latent in salivary glands and saliva is the main form of viral transmission. The objective of this study was to assess HHV6 incidence in the fluids from healthy individuals using a standardised technique for collecting and extracting viral DNA from gingival. crevicular fluid, whole saliva and parotid gland saliva. Design: Samples of oral fluids and peripheral. blood were collected from 28 blood donors and HHV6 was detected using PCR assay. Parotid gland saliva and gingival crevicular fluid were collected by endodontic paper cones in order to not contaminate these fluids with whole saliva. Results: Of the 28 donors, 20 (71.4%) presented positive results in at least one of the three oral fluids researched. Whole saliva was positive in 19 (67.8%) volunteers, while only four (14.2%) samples of gingival crevicular fluid and four of parotid gland saliva proved to be positive. Conclusions: The results suggest that HHV6 is present in the saliva of a large proportion of the healthy adult population. The use of endodontic paper cones for oral fluid collection and viral extraction was efficient, simple, cheap and painless. In spite of, the small number of cases studied it was possible to demonstrate that neither gingival crevicular fluid nor parotid gland saliva were the principal source of HHV6 in whole saliva. (C) 2004 Elsevier Ltd. All rights reserved.