Complete mitochondrial genomes of two Japanese precious corals, Paracorallium japonicum and Corallium konojoi (Cnidaria, Octocorallia, Coralliidae) : Notable differences in gene arrangement
Complete mitochondrial genomes of two Japanese precious corals, Paracorallium japonicum and Corallium konojoi (Cnidaria, Octocorallia, Coralliidae) : Notable differences in gene arrangement
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两种日本珍贵珊瑚 Paracorallium japonicum 和 Corallium konojoi (Cnidaria, Octocorallia, Coralliidae) 的完整线粒体基因组：基因排列的显着差异
作者：
Uda K.;Komeda Y.;Koga K.;Fujita T.;Iwasaki N.;Suzuki;T
珍贵的珊瑚在分类学上是一组珊瑚，属于Alcyonacea，Subclass Corallia和Anthozoa的珊瑚科，其骨骼轴用于珠宝。分布于地中海及日本、台湾、中途岛和夏威夷群岛附近的沃茨。珊瑚科的珊瑚属最近被分为两个属，Corallium和Paracorallium，基于形态学观察，但迄今为止还没有足够的分子证据支持这一分类。首次测定了两种珍贵珊瑚P. japonicum和C. konojoi，以澄清其系统的立场。日本对虾和中国对虾的环状线粒体基因组。konojoi基因全长分别为18，913 bp和18，969 bp，编码1 - 3个典型的能量途径蛋白编码基因（nad 1 -6、nad 4L、cox 1 -3、cob、atp 6和atp 8）、两个核糖体RNA基因（rns和rnl）、一个转运RNA（trnM）和一个错配修复基因同源物msh 1。这两个基因组的核苷酸序列同源性为97.5%，这与Acanella eburnea和Keratoisidinae sp.之间的同源性相当。然而，令人惊讶的是，它们的基因排列并不相同。系统发育分析，使用7个完整的线粒体基因组序列属于亚类中的珊瑚虫的物种表明，在该子类中，至少有三个基因顺序重排事件发生在进化过程中。我们的研究结果支持的形态分类的有效性，将科珊瑚分为两个属，Corallium和Paracorallium。
Precious coral are taxonomically a group of corals that belong to the family Coralliidae within the order Alcyonacea, subclass Octocorallia, and class Anthozoa, whose skeletal axes are used for jewelry. They are distributed in the Mediterranean Sea and in waters adjacent to Japan, Taiwan, Midway Island and the Hawaiian Islands. The genus Corallium of the family Coralliidae was recently divided into two genera, Corallium and Paracorallium, based on morphological observations, but insufficient molecular evidence to support this classification has been presented to date. We determined for the first time the complete mitochondrial genome sequence of two precious corals P. japonicum and C. konojoi, in order to clarify their systematic positions. The circular mitochondrial genomes of P. japonicum and C. konojoi are 18,913bp and 18,969bp in length, respectively, and encode 13 typical energy pathway protein coding genes (nad1–6, nad4L, cox1–3, cob, atp6 and atp8), two ribosomal RNA genes (rns and rnl), a transfer RNA (trnM) and a mismatch repair gene homologue msh1. The two genomes have an overall nucleotide sequence identity of 97.5%, which is comparable to that between Acanella eburnea and Keratoisidinae sp. belonging to Octocorallia. Surprisingly, however, their gene arrangements were not identical. Phylogenetic analyses using seven complete mitochondrial genome sequences belonging to species in the subclass Octocorallia indicated that within the subclass, at least three gene order rearrangement events occurred during evolution. Our results support the validity of the morphological classification that separated the family Coralliidae into two genera, Corallium and Paracorallium.