Periodontal Regeneration Using Cultured Coral Scaffolds in Class II Furcation Defects in Dogs
Periodontal Regeneration Using Cultured Coral Scaffolds in Class II Furcation Defects in Dogs
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DOI：
10.2485/jhtb.28.329
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发表时间：
2019-01-01
影响因子：
0.4
通讯作者：
Hashimoto, Yoshiya
中科院分区：
文献类型：
--
作者：
Matuda, Yoshifumi;Okamura, Tomoharu;Hashimoto, Yoshiya
自体骨移植涉及二次手术，并受到可收集骨量的限制。作为骨移植的商业珊瑚产品是由自然形态的珊瑚组成的。建议将人工培育的珊瑚作为替代品，以克服自然珊瑚灭绝的威胁。本研究的目的是探讨培养的珊瑚支架在犬牙周组织再生修复II类分叉缺损中的作用。采用扫描电子显微镜(SEM)、粉末X射线衍射仪(XRD)和傅立叶变换红外光谱(FTIR)对培养的珊瑚进行了表征。选用2只雌性比格犬的12颗下颌前磨牙。每只犬左下颌前磨牙3颗分叉缺损处植入培养的珊瑚。对照组由每只狗的右侧下颌前磨牙组成，在分叉缺损处不接受种植体材料。术后8周处死动物，用组织学和放射学方法进行组织学和放射学分析。扫描电子显微镜显示为三维陶瓷结构，孔径在50-250 mm之间。培养的珊瑚颗粒表面覆盖着许多粗糙的、多孔的突起。X-射线衍射仪和傅里叶变换红外光谱分析表明，培养的珊瑚同时存在文石和方解石两种相，称为二相性。种植到缺损处的培养珊瑚颗粒被完全吸收，并被具有哈弗斯结构或没有任何炎症反应的骨取代。连接新生骨和牙骨质的组织良好的牙周韧带被再生到分叉间隙的顶部。组织病理学评估表明，培养的珊瑚支架具有再生牙周组织的潜力，在狗的II类分叉缺损中。
Autogenous bone grafting involves a second surgery and is limited by the availability of collectable bone. Coral products commercially available as bone grafts are composed of corals in their natural form. Cultured corals are recommended as a substitute to overcome the threat of extinction of natural corals. The purpose of this study was to investigate the potential of cultured coral scaffolds for periodontal tissue regeneration in class II furcation defects in dogs. The cultured coral used for this study was characterized using scanning electron microscopy (SEM), powder X-ray diffraction (XRD) and Fourier-transform infrared (FTIR) spectroscopy. Twelve mandibular premolar teeth from two female beagle dogs were used. Furcation defects of three left mandibular premolar teeth of each dog received the cultured coral. A control group consisting of the right mandibular premolar teeth from each dog received no implant material within the furcation defects. The dogs were then sacrificed 8 weeks post-surgery, and healing was evaluated histologically and radiologically using micro-computed tomography analysis. SEM micrographs showed a tri-dimensional ceramic structure with pore size ranging 50-250 mm. The surface of the cultured coral particles was covered with numerous rough, porous processes. XRD and FTIR results showed that the cultured coral exhibited both aragonite and calcite phases, referred to as dimorphism. The cultured coral particles implanted into the defects were completely resorbed and replaced by bone with either a Haversian structure or without any inflammatory reaction. A well-organized periodontal ligament bridging the new bone and cementum was regenerated to the top of the furcation space. Histopathological evaluation suggests that cultured coral scaffolds have the potential to regenerate periodontal tissue in class II furcation defects in dogs.