Extracellular matrix components influence the survival of adult cardiac myocytes in vitro.
Extracellular matrix components influence the survival of adult cardiac myocytes in vitro.
复制标题
DOI：
10.1016/0014-4827(85)90462-8
复制
发表时间：
1985-06
影响因子：
3.7
通讯作者：
E. Lundgren;E. Lundgren;L. Terracio;S. Mårdh;T. Borg
中科院分区：
文献类型：
--
作者：
E. Lundgren;E. Lundgren;L. Terracio;S. Mårdh;T. Borg
采用胶原酶灌注法从成年大鼠心脏中分离出耐钙心肌细胞，并在无血清培养基中涂于不同底物上，观察其与不同细胞外基质（ECM）组分的粘附性。肌细胞很容易附着在涂有IV型胶原蛋白（C-IV）、层粘连蛋白（LN）和胎牛血清（FBS）的培养皿上，附着方式取决于这些成分的浓度。在涂有纤维连接蛋白（FN）的培养皿或未涂有纤维连接蛋白的塑料培养皿上粘附的肌细胞明显减少。细胞在1-4小时内同样粘附在涂有C-IV、LN和FBS的培养皿上。然而，在培养2周后检查时，发现只有C-IV和LN可以支持附着的肌细胞的存活，当在C-IV或LN上培养时，肌细胞扩散并形成致密的单层。此时肌动蛋白丝沿细胞长轴线性重组，肌细胞自发收缩。制备兔肌细胞膜抗体，并研究其抑制ECM成分附着的能力。纯化的IgG抑制对C-IV的附着，而对LN的附着只有轻微的影响。这些数据与与ECM底物相互作用并影响细胞形状并可能因此影响细胞功能的特定细胞表面组分的存在相一致。
Calcium-tolerant myocytes were isolated from adult rat hearts by collagenase perfusion and plated on various substrates in serum-free medium and their adhesion to various extracellular matrix (ECM) components was determined. The myocytes attached readily to dishes coated with collagen type IV (C-IV), laminin (LN), and to fetal bovine serum (FBS) in a manner dependent on the concentration of the components. Substantially fewer myocytes adhered to dishes coated with fibronectin (FN) or to uncoated plastic dishes. Cells adhered equally well to dishes coated with C-IV, LN and FBS within 1–4 h. However, when examined after 2 weeks in culture it was found that only C-IV and LN could support survival of the attached myocytes, and when cultured on C-IV or LN the myocytes were spread and had formed a dense monolayer. The actin filaments had at this time reorganized linearly along the long axis of the cell and the myocytes contracted spontaneously. Rabbit antibodies were raised against myocyte membranes and their ability to inhibit attachment to ECM components was studied. Purified IgG inhibited attachment to C-IV, while having only a minor effect on attachment to LN. These data are compatible with the presence of a specific cell surface component(s) that interacts with ECM substrates and influences cell shape and possibly thereby influences cellular functions.