The AP2-type transcription factors DORNRÖSCHEN and DORNRÖSCHEN-LIKE promote G1/S transition
The AP2-type transcription factors DORNRÖSCHEN and DORNRÖSCHEN-LIKE promote G1/S transition
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DOI：
10.1007/s00438-016-1224-x
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发表时间：
2016-06
影响因子：
3.1
通讯作者：
Ingo Seeliger;Anneke Frerichs;Dorothea Glowa;Laura Velo;Petra Comelli;J. Chandler;W. Werr
中科院分区：
文献类型：
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作者：
Ingo Seeliger;Anneke Frerichs;Dorothea Glowa;Laura Velo;Petra Comelli;J. Chandler;W. Werr
旁系同源基因DORNRÖSCHEN（DRN）和DORNRÖSCHEN-LIKE（DRNL）编码AP 2型转录因子，这些转录因子在拟南芥茎分生组织（SAM）的中央干细胞区或外周区的侧向器官建立细胞（LOFC）中表达并自主作用，但它们的分子作用尚不清楚。在这里，我们显示使用拟南芥MERISTEM LAYER 1启动子，DRN和DRN L在细胞周期进程中具有共同的功能，并可能为SAM中的G1-S转换提供局部能力。对双转基因DRN：：erGFP和DRNL：：erCERULEAN启动子融合系的分析表明，这种细胞能力的轨迹始于中央干细胞区的DRN活性，并通过DNL活性局部延伸到IM或FM周边的创始细胞群。我们的数据支持的情况下，基因复制后，DRN和DRN L获得不同的转录结构域内的拍摄分生组织，但保留蛋白质的功能，影响细胞周期的进展，无论是中央在干细胞或周边的原始创始人细胞，一个发现是分生组织功能的一般相关性。
The paralogous genesDORNRÖSCHEN(DRN) andDORNRÖSCHEN-LIKE(DRNL) encode AP2-type transcription factors that are expressed and act cell-autonomously in the central stem-cell zone or lateral organ founder cells (LOFCs) in the peripheral zone of the Arabidopsis shoot meristem (SAM), but their molecular contribution is unknown. Here, we show using theArabidopsis thaliana MERISTEM LAYER 1promoter thatDRNandDRNLshare a common function in cell cycle progression and potentially provide local competence for G1-S transitions in the SAM. Analysis of double transgenicDRN::erGFPandDRNL::erCERULEANpromoter fusion lines suggests that the trajectory of this cellular competence starts withDRNactivity in the central stem-cell zone and extends locally viaDRNLactivity into groups of founder cells at the IM or FM periphery. Our data support the scenario that after gene duplication,DRNandDRNLacquired different transcription domains within the shoot meristem, but retained protein function that affects cell cycle progression, either centrally in stem cells or peripherally in primordial founder cells, a finding that is of general relevance for meristem function.