The first identification of rotavirus B from children and adults with acute diarrhoea in kathmandu, Nepal.
The first identification of rotavirus B from children and adults with acute diarrhoea in kathmandu, Nepal.
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DOI：
10.2149/tmh.2013-15
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发表时间：
2013-09
影响因子：
4.5
通讯作者：
Pandey BD
中科院分区：
文献类型：
--
作者：
Alam MM;Pun SB;Gauchan P;Yokoo M;Doan YH;Tran TN;Nakagomi T;Nakagomi O;Pandey BD
已知呼肠孤病毒科轮状病毒属中的轮状病毒B（RVB）是包括中国、印度、孟加拉国和缅甸在内的亚洲部分地区儿童和成人急性胃肠炎的病因。在2007年3月至2008年5月的15个月监测计划中，在尼泊尔加德满都的一家传染病医院从患有急性胃肠炎的儿童和成人中收集了3，080个粪便样本。在25个（0.8%）标本中检测到RVB，在尼泊尔首次，通过使用聚丙烯酰胺凝胶电泳，然后用逆转录PCR和测序确认。本研究中检测到的菌株具有非常相似的电泳分型，它们的VP 7序列几乎相同，并且在遗传学上属于印度-孟加拉谱系，而不同于中国谱系。因此，这项研究表明，属于印度-孟加拉国血统的RVB菌株在比以前记录的更广泛的区域中传播，这表明这种系统发育划分与喜马拉雅山脉造成的地理划分相对应。越南北方与中国南方接壤，边境线较长，山区较少，因此有必要进行进一步的研究，以鉴定和表征越南北部的RVB菌株。
Rotavirus B (RVB) in the genus Rotavirus of the family Reoviridae is known to be a cause of acute gastroenteritis among children and adults in parts of Asia including China, India, Bangladesh and Myanmar. In a 15-month surveillance programme between March 2007 and May 2008, 3,080 stool specimens were collected from children and adults with acute gastroenteritis in an infectious disease hospital in Kathmandu, Nepal. In 25 (0.8%) specimens RVB was detected, for the first time in Nepal, by the use of polyacrylamide gel electrophoresis followed by confirmation with reverse-transcription PCR and sequencing. The strains detected in this study had very similar electropherotypes, and their VP7 sequences were almost identical and phylogenetically belonged to the Indo-Bangladeshi lineage which was distinct from the Chinese lineage. Thus, this study showed the circulation of RVB strains belonging to the Indo-Bangladeshi lineage in a broader region than previously documented, suggesting that this phylogenetic divide corresponded to the geographic divide created by the Himalayan Mountains. Further studies may be warranted to identify and characterize the RVB strains in northern Vietnam which is adjacent to southern China with a long and less mountainous border.