The Development of the Enteropneust Hemichordate Balanoglossus misakiensis Kuwano
The Development of the Enteropneust Hemichordate Balanoglossus misakiensis Kuwano
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川野肠半索动物 Balanoglossus misakiensis 的发育
DOI：
10.2108/zsj.21.533
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发表时间：
2004
期刊：
影响因子：
--
通讯作者：
M. Yamaguchi
中科院分区：
文献类型：
--
作者：
M. Urata;M. Yamaguchi
摘要本文描述了味色海鞘从受精到变态的发育过程。这是第一个描述在实验室条件下间接发育的半脊髓的完全发育的报告。将海水温度从23℃调至28℃，可诱导成熟成虫产卵。卵直径200μm，包裹在非粘性膜内，在海水中很容易分散。人工授精后，受精包膜从卵膜下的卵面隆起，随后在包膜内形成第一和第二极体。受精卵每隔20分钟进行一次卵裂，形成囊胚，受精后9小时开始原肠发育。胚胎在受精后1天孵化，成为典型的摄食性旋毛虫幼虫。幼虫在受精后7-10天变态，未经历间接发育的第一阶段(Müler)或第四阶段(Krohn)。没有摄食的幼虫未能变态。幼体在培养管底部的沙子中定居一周内完成成体形成。我们讨论了味佐氏假单胞菌发育的异时性修改，并将该物种作为研究间接发育的半角类动物的潜在模式生物。
Abstract We describe development from fertilization to metamorphosis of the enteropneust hemichor-date Balanoglossus misakiensis. This is the first report to describe the complete development of an indirect-developing hemichordate under laboratory conditions. Mature adults were induced to spawn by shifting the temperature of seawater from 23 to 28°C. Eggs (200 μm diameter) were enclosed within a nonmucilaginous membrane, and dispersed readily in seawater. After artificial insemination, a fertilization envelope was elevated from the egg surface beneath the egg membrane; this was followed by the formation of the first and second polar bodies within the envelope. Zygotes cleaved at 20-min intervals to form blastulae, and gastrulation started 9 h after fertilization. Embryos hatched 1 day after fertilization to become typical feeding tornaria larvae. The larvae metamorphosed 7–10 days after fertilization without undergoing the first (Müller) or forth (Krohn) stage of indirect-developing hemichordate development. Larvae that were not fed failed to metamorphose. Juveniles completed adult body formation within a week of settling in sand at the bottom of the culture tube. We discuss heterochronical modifications of B. misakiensis development, and make the case for this species as a potential model organism for the investigation of indirect-developing hemichordates.
影响因子：
2.7
作者：
K. Peterson;R. Cameron;E. Davidson
通讯作者：
K. Peterson;R. Cameron;E. Davidson