Prime-boost immunization with DNA followed by a recombinant vaccinia virus expressing P50 induced protective immunity against Babesia gibsoni infection in dogs
Prime-boost immunization with DNA followed by a recombinant vaccinia virus expressing P50 induced protective immunity against Babesia gibsoni infection in dogs
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DOI：
10.1016/j.vaccine.2006.09.083
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发表时间：
2007-01-26
期刊：
影响因子：
5.5
通讯作者：
Xuan, Xuenan
中科院分区：
文献类型：
--
作者：
Fukumoto, Shinya;Tamaki, Yoh;Xuan, Xuenan
研究表明，以DNA为起始DNA，再以表达相关抗原的重组痘苗病毒为基础的异种免疫方案，能有效地诱导对多种感染性病原体的免疫应答。在本研究中，我们构建了表达吉氏巴贝斯虫P50的重组表达载体和痘苗病毒，研究了异种免疫的免疫原性和保护效果。与对照组相比，加强免疫组的P50抗体水平显著升高，重组痘苗病毒加强免疫后抗体水平显著升高。加强免疫诱导犬产生特异性IgG2抗体应答和干扰素-γ的产生。用表达P50的重组痘苗病毒加强免疫2周后，用吉氏杆菌帕塔斯体攻击。用加强免疫方案免疫的狗表现出部分保护作用，表现为寄生虫血症水平显著降低，最高寄生虫血症延迟2天。这些结果表明，这种异种加强免疫方法可能对预防犬吉氏杆菌感染有效。(C)2006爱思唯尔有限公司。保留所有权利。
A heterologous prime-boost immunization regime with priming DNA followed by recombinant vaccinia virus expressing relevant antigens has been shown to induce effective immune responses against several infectious pathogens. In this study, we constructed a recombinant plasmid and vaccinia virus, both of which expressed P50 of Babesia gibsoni, to investigate the immunogenicity and protective efficacy of a heterologous prime-boost immunization against canine babesiosis. The dogs immunized with the prime-boost regime developed a significantly high level of specific antibody against P50 when compared with the control groups, and the antibody level was strongly increased after a booster immunization with a recombinant vaccinia virus. The prime-boost immunization regime induced a specific IgG2 antibody response and IFN-gamma production in dogs. Two weeks after the booster immunization with a recombinant vaccinia virus expressing P50, the dogs were challenged with B. gibsoni patasites. The dogs immunized with the prime-boost regime showed partial protection, manifested as a significantly low level of parasitemia and a 2-day delay of the peak parasitemia. These results indicated that such a heterologous prime-boost immunization approach might be useful against B. gibsoni infection in dogs. (c) 2006 Elsevier Ltd. All rights reserved.