Disposition of D-[U-14C]tagatose in the rat
Disposition of D-[U-14C]tagatose in the rat
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DOI：
10.1006/rtph.1998.1251
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发表时间：
1999-04-01
影响因子：
3.4
通讯作者：
Levin, GV
中科院分区：
文献类型：
--
作者：
Saunders, JP;Zehner, LR;Levin, GV
本实验的目的是确定D-塔格糖作为一种低热量甜味剂在常规和无菌雄性大鼠中的分布。一组常规大鼠喂食含有D-塔格糖（100 g/kg）的饮食与未纯化饮食（900 g/kg）混合28天。然后，将[U-C-14]-标记的D-塔格糖以单剂量（约220-380 kBq）施用给这些适应大鼠中的4只，以及15只先前没有暴露的常规和无菌大鼠（即，将19只给药动物中的11只（4只适应的常规、3只不适应的常规和2只不适应的无菌，均经口给药，加上2只不适应的常规静脉内给药）置于代谢室中，并定期采集CO2、尿液和粪便样品。在终止时，根据C-14的回收率获得了完整的物料平衡。在6小时的消化期内，D-塔格糖代谢释放39.9和13.9%的口服剂量的CO2在适应的常规大鼠和不适应的无菌大鼠，分别。总释放量分别约为68%和22%。CO2释放的差异归因于D-塔格糖在常规大鼠肠道中的微生物发酵。通过发现适应的常规大鼠的粪便中的D-塔格糖比未适应的常规大鼠的粪便中的D-塔格糖少93%，证实了适应的作用。D-塔格糖在大鼠体内的肠道吸收估计为20%。结果表明，D-塔格糖主要由大鼠肠道中的微生物代谢，宿主代谢的上限为口服剂量的15 - 20%。(C)北京：科学出版社.
The purpose of this experiment was to determine the disposition of D-tagatose, under development as a low-calorie sweetener, in conventional and germ-free male rats. One group of conventional rats was fed a diet containing D-tagatose (100 g/kg) mixed with the nonpurified diet (900 g/kg) for 28 days. Then, [U-C-14]-labeled D-tagatose was administered as a single dose (approximately 220-380 kBq) to 4 of these adapted rats, as well as to 15 conventional and germ-free rats with no prior exposure (i.e., unadapted) to D-tagatose, Eleven of the 19 dosed animals (4 adapted conventional, 3 unadapted conventional and 2 unadapted germ-free, all dosed orally, plus 2 unadapted conventional dosed intravenously) were placed in metabolism chambers and samples of CO2, urine, and feces taken at regular intervals. At termination, a complete material balance was obtained based on the recovery of C-14. Over the 6-h digestive period, D-tagatose was metabolized to release 39.9 and 13.9% of the oral dose as CO2 in the adapted conventional rats and in the unadapted germ-free rats, respectively. Total releases approximated 68 and 22%, respectively. The difference in CO2 evolution is ascribed to microbial fermentation of D-tagatose in the gut of the conventional rats. The role of adaptation was confirmed by finding 93% less D-tagatose in the feces of the adapted conventional rat than in the feces of the unadapted conventional rat. The intestinal absorption of D-tagatose in the rat is estimated to be 20%. The results demonstrate that D-tagatose is metabolized primarily by microorganisms in the gut of the rat, with an upper limit between 15 and 20% of oral dose metabolized by the host. (C) 1999 Academic Press.