MICROBIAL TRANSFORMATIONS OF FERULIC ACID BY SACCHAROMYCES-CEREVISIAE AND PSEUDOMONAS-FLUORESCENS
MICROBIAL TRANSFORMATIONS OF FERULIC ACID BY SACCHAROMYCES-CEREVISIAE AND PSEUDOMONAS-FLUORESCENS
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DOI：
10.1128/aem.59.7.2244-2250.1993
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发表时间：
1993-07-01
影响因子：
4.4
通讯作者：
ROSAZZA, JPN
中科院分区：
文献类型：
--
作者：
HUANG, ZX;DOSTAL, L;ROSAZZA, JPN
酿酒酵母（干面包酵母）和荧光假单胞菌被用来转化反式阿魏酸为4-羟基-3-甲氧基苯乙烯，产率分别为96%和89%。代谢产物通过固相萃取分离，并通过薄层色谱法和高效液相色谱法进行分析。通过H-1-和C-13-核磁共振光谱法和质谱法确定代谢产物的身份。阿魏酸脱羧的机理进行了研究，通过测量的程度和位置的氘纳入苯乙烯衍生物从D2 O通过质谱和质子和氘核磁共振光谱。休息细胞的面包酵母减少阿魏酸4-羟基-3-甲氧基苯基丙酸在54%的产率时，孵育在氩气气氛下。
Saccharomyces cerevisiae (dry baker's yeast) and Pseudomonas fluorescens were used to convert trans-ferulic acid into 4-hydroxy-3-methoxystyrene in 96 and 89% yields, respectively. The metabolites were isolated by solid-phase extraction and analyzed by thin-layer chromatography and high-performance liquid chromatography. The identities of the metabolites were determined by H-1- and C-13-nuclear magnetic resonance spectroscopy and by mass spectrometry. The mechanism of the decarboxylation of ferulic acid was investigated by measuring the degree and position of deuterium incorporated into the styrene derivative from D2O by mass spectrometry and by both proton and deuterium nuclear magnetic resonance spectroscopies. Resting cells of baker's yeast reduced ferulic acid to 4-hydroxy-3-methoxyphenylpropionic acid in 54% yield when incubations were under an argon atmosphere.