Transcription factors Sox10 and Sox2 functionally interact with positive transcription elongation factor b in Schwann cells
Transcription factors Sox10 and Sox2 functionally interact with positive transcription elongation factor b in Schwann cells
复制标题
DOI：
10.1111/jnc.13013
复制
发表时间：
2015-02-01
影响因子：
4.7
通讯作者：
Wegner, Michael
中科院分区：
文献类型：
--
作者：
Arter, Juliane;Wegner, Michael
Sox蛋白是一种多功能的调节因子，与不同的发育过程相关，对染色质结构、DNA构象和转录起始有重要影响。在这里，我们表明，Sox 2和Sox 10，两个重要的Sox蛋白的雪旺细胞的发展，也有能力激活转录延伸的雪旺细胞系通过招聘的正转录延伸因子B。招募介导的两个Sox蛋白和细胞周期蛋白T1亚基的正转录延伸因子B的羧基末端的反式激活结构域之间的物理相互作用，与两个Sox蛋白的相互作用界面被映射到细胞周期蛋白T1的中央部分的相邻区域。支持这种相互作用的雪旺细胞发育的相关性，髓磷脂基因的转录出现在伸长水平的调节。因此，我们的研究结果增加了一个新的方面的活动的Sox蛋白和扩展的功能库，这一重要的发展调节。
Sox proteins are mechanistically versatile regulators with established relevance to different developmental processes and crucial impact on chromatin structure, DNA conformation, and transcriptional initiation. Here, we show that Sox2 and Sox10, two Sox proteins important for Schwann cell development, also have the capability to activate transcriptional elongation in a Schwann cell line by recruiting the positive transcription elongation factor b. Recruitment is mediated by physical interaction between the carboxyterminal transactivation domains of the two Sox proteins and the Cyclin T1 subunit of positive transcription elongation factor b, with interaction interfaces for the two Sox proteins being mapped to adjacent regions of the central part of Cyclin T1. Supporting the relevance of this interaction to Schwann cell development, transcription of myelin genes appears regulated at the level of elongation. Our results thus add a new facet to the activity of Sox proteins and expand the functional repertoire of this important group of developmental regulators.