Short interfering RNA (siRNA)-mediated RNA interference (RNAi) in human cells
Short interfering RNA (siRNA)-mediated RNA interference (RNAi) in human cells
复制标题
DOI：
10.1196/annals.1281.007
复制
发表时间：
2003-01-01
期刊：
影响因子：
--
通讯作者：
Mousses, S
中科院分区：
文献类型：
--
作者：
Caplen, NJ;Mousses, S
哺乳动物细胞中的瞬时基因沉默可以由类似于20 -25个核苷酸的双链RNA（dsRNA）分子介导，称为短干扰RNA（siRNA）。在低等真核生物中天然存在的siRNA具有特征性的结构元件;然而，对于外源siRNA在哺乳动物中介导RNAi的关键特征知之甚少。我们最近确定了影响哺乳动物细胞中siRNA介导的RNA干扰效率的一些关键参数，并一直在考虑使用RNA干扰作为功能基因组学工具，特别是用于高通量分析，以及RNA干扰作为治疗工具的潜在用途。
Transient gene silencing in mammalian cells can be mediated by double stranded RNA (dsRNAs) molecules of similar to20-25 nucleotides termed short interfering (siRNAs). Naturally occurring siRNAs in lower eukaryotes have characteristic structural elements; however, little is known about what features are critical for an exogenous siRNA to mediate RNAi in mammals. We have recently determined some of the critical parameters that influence the efficiency of siRNA-mediated RNAi in mammalian cells and have been considering the use of RNAi as a functional genomics tool, particularly for high throughput analysis, and the potential use of RNAi as a therapeutic tool.