A global census of nitrogenase diversity
A global census of nitrogenase diversity
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DOI：
10.1111/j.1462-2920.2011.02488.x
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发表时间：
2011-07-01
影响因子：
5.1
通讯作者：
Buckley, Daniel H.
中科院分区：
文献类型：
--
作者：
Gaby, John Christian;Buckley, Daniel H.
通过构建和分析16 989个nifH序列的比对数据库，对固氮微生物的全球多样性进行了评估。我们的结论是，固氮生物的多样性仍然是描述不佳，许多生物仍有待发现。我们的分析表明，多样性是不均匀分布在系统发育组或跨环境，一些最多样化的组合和环境仍然是最差的特点。大多数OTU是罕见的，落在频率分布的长尾中。最主要的OTU属于蓝细菌或α、β和γ变形菌，并且这些主要OTU中的五个没有任何代表性的分离培养。土壤中含有最大的多样性的nifH序列的所有环境调查。集群III，这是占主导地位的专性厌氧菌的nifH序列，被发现包含最大的多样性的所有nifH谱系，也是一组的多样性是最少的采样。我们的研究结果提供了正在进行的努力，探索固氮生物多样性的背景下，表明特定的群体和环境，仍然很差的特点。
The global diversity of nitrogen-fixing microorganisms was assessed through construction and analysis of an aligned database of 16 989 nifH sequences. We conclude that the diversity of diazotrophs is still poorly described and that many organisms remain to be discovered. Our analyses indicate that diversity is not distributed evenly across phylogenetic groups or across environments and that some of the most diverse assemblages and environments remain the most poorly characterized. The majority of OTUs were rare, falling in the long tail of the frequency distribution. The most dominant OTUs fell into either the Cyanobacteria or the alpha, beta, and gamma Proteobacteria, and five of these dominant OTUs do not have any representatives cultivated in isolation. Soils contained the greatest diversity of nifH sequences of all of the environments surveyed. Cluster III, which is dominated by nifH sequences from obligate anaerobes, was found to contain the greatest diversity of all nifH lineages and is also the group for which diversity is the least sampled. Our findings provide context for ongoing efforts to explore diazotroph diversity, indicating specific groups and environments that remain poorly characterized.