ncHMR detector: a computational framework to systematically reveal non-classical functions of histone modification regulators
ncHMR detector: a computational framework to systematically reveal non-classical functions of histone modification regulators
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DOI：
10.1186/s13059-020-01953-0
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发表时间：
2020-02-24
期刊：
影响因子：
12.3
通讯作者：
Zhang, Yong
中科院分区：
文献类型：
--
作者：
Hu, Shengen;Huo, Dawei;Zhang, Yong
近年来，组蛋白修饰调节因子（HMR）的一些非经典功能，独立于其已知的组蛋白修饰底物和产物，已被报道是必不可少的特定的细胞过程。然而，没有为系统地确定这些职能而设计的框架。在这里，我们开发了ncHMR检测器，这是第一个基于ChIP-seq数据挖掘来预测给定HMR的非经典函数和辅因子的计算框架。我们将ncHMR检测器应用于ChIP-seq数据丰富的细胞类型，并预测HMR的非经典功能。最后，我们通过实验揭示了预测的CBX 7的非经典功能对于维持多能性具有生物学意义。
Recently, several non-classical functions of histone modification regulators (HMRs), independent of their known histone modification substrates and products, have been reported to be essential for specific cellular processes. However, there is no framework designed for identifying such functions systematically. Here, we develop ncHMR detector, the first computational framework to predict non-classical functions and cofactors of a given HMR, based on ChIP-seq data mining. We apply ncHMR detector in ChIP-seq data-rich cell types and predict non-classical functions of HMRs. Finally, we experimentally reveal that the predicted non-classical function of CBX7 is biologically significant for the maintenance of pluripotency.