Modification of the phenotypic expression of murine dystrophy: a morphological study.
Modification of the phenotypic expression of murine dystrophy: a morphological study.
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小鼠营养不良表型表达的修饰：形态学研究。
DOI：
10.1002/ar.1092140104
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
Ontell,M
中科院分区：
文献类型：
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作者：
Bourke,DL;Ontell,M
将4~6周龄正常小鼠(129Rej)和营养不良小鼠(129Rej dy/dy)的指长伸肌进行原位移植。分别于移植后1、3、7、14、20、50、100天对移植肌肉进行检查。两种移植物的肌纤维经历了相似的坏死和再生过程。除了最初的坏死反应，在两种类型的移植肌肉中都没有发现坏死肌纤维的证据。在移植后100天，移植的正常和营养不良的肌肉基本上是相似的，除了营养不良的移植更小。根据100d移植物最宽周长的肌纤维数目的比较[正常移植物为631±59(Bourke and Ontel，1984)；营养不良移植物为631±74，营养不良移植物]，我们认为4-6周龄营养不良肌肉的再生能力与创伤正常肌肉相似。在移植后100天，移植的营养不良肌肉比年龄匹配的营养不良小鼠的未损伤肌肉看起来更健康，肌纤维直径变化较小，更好的束状组织和较少的结缔组织。该移植系统展示了使用环境操作改变肌病遗传编程表达的可能性。
The extensor digitorum longus muscles of 4–6‐week‐old normal mice (129 ReJ) and dystrophic mice (129 ReJ dy/dy) were orthotopically transplanted. Grafted muscles were examined 1, 3, 7, 14, 20, 50, and 100 days post‐transplantation. The myofibers of both types of grafts underwent a similar time course of necrosis and regeneration. Other than during the initial necrotic response, no evidence of necrotic myofibers was found in either type of grafted muscle. At 100 days post‐transplantation, the grafted normal and dystrophic muscles were essentially similar, except that the dystrophic graft was of smaller size. Based on a comparison of the number of myofibers found at the 100‐day grafts' widest girths [631 ± 59 SEM, for normal grafts (Bourke and Ontell, 1984); 631 ± 74 SEM, for dystrophic grafts], it is suggested that the regenerative capability of traumatized 4–6‐week‐old dystrophic muscle is similar to that of traumatized normal muscle. At 100 days post‐transplantation, the grafted dystrophic muscle appeared “healthier” than untraumatized muscle from age‐matched dystrophic mice, having less variation in myofiber diameter, better fascicular organization, and less connective tissue. The transplantation system demonstrates the possibility of modifying the expression of genetic programming of myopathic disorders using environmental manipulation.