Maternal circadian disruption is associated with variation in placental DNA methylation
Maternal circadian disruption is associated with variation in placental DNA methylation
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DOI：
10.1371/journal.pone.0215745
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发表时间：
2019-04-26
期刊：
影响因子：
3.7
通讯作者：
Marsit, Carmen J.
中科院分区：
文献类型：
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作者：
Clarkson-Townsend, Danielle A.;Everson, Todd M.;Marsit, Carmen J.
昼夜节律紊乱是一种常见的环境和职业暴露，具有公共卫生后果，但对昼夜节律紊乱是否影响子宫内发育知之甚少。我们在一项针对夜班工作的表观基因组关联研究（EWAS）中，以夜班工作为代表，调查了母体昼夜节律紊乱是否与胎盘组织DNA甲基化模式的变化相关。在这里，我们比较了胎盘组织中胞嘧啶-鸟苷二核苷酸（CpG）特异性甲基化的全基因组，（用Illumina 450 K阵列测量）来自罗得岛儿童健康研究（RICHS）的参与者（n = 237），他们报告（n = 53）和没有报告（n = 184）上夜班，使用稳健的线性模型并调整母亲年龄，孕前吸烟，婴儿性别，母亲的不幸，以及假定的细胞混合物。对多重比较进行了统计分析调整，并对错误发现率进行了Bonferroni或Benjamini和Hochberg（BH）调整。夜班工作与胎盘组织中的差异甲基化相关，包括基因NAV 1、SMPD 1、TAPBP、CLEC 16 A、DIP 2C、FAM 172 A和PLEKHG 6中的CpG位点（Bonferroni校正p
Circadian disruption is a common environmental and occupational exposure with public health consequences, but not much is known about whether circadian disruption affects in utero development. We investigated whether maternal circadian disruption, using night shift work as a proxy, is associated with variations in DNA methylation patterns of placental tissue in an epigenome-wide association study (EWAS) of night shift work. Here, we compared cytosine-guanosine dinucleotide (CpG) specific methylation genome-wide of placental tissue (measured with the Illumina 450K array) from participants (n = 237) in the Rhode Island Child Health Study (RICHS) who did (n = 53) and did not (n = 184) report working the night shift, using robust linear modeling and adjusting for maternal age, pre-pregnancy smoking, infant sex, maternal adversity, and putative cell mixture. Statistical analyses were adjusted for multiple comparisons and results presented with Bonferroni or Benjamini and Hochberg (BH) adjustment for false discovery rate. Night shift work was associated with differential methylation in placental tissue, including CpG sites in the genes NAV1, SMPD1, TAPBP, CLEC16A, DIP2C, FAM172A, and PLEKHG6 (Bonferroni-adjusted p