Infertility caused by hCG autoantibody
Infertility caused by hCG autoantibody
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DOI：
10.1210/jc.87.3.993
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发表时间：
2002-03-01
影响因子：
5.8
通讯作者：
Norman, RJ
中科院分区：
文献类型：
--
作者：
Amato, F;Warnes, GM;Norman, RJ
在一名有9年继发性不孕病史的患者体内发现了一种抗人绒毛膜促性腺激素（hCG）自身抗体。尽管该患者月经周期规律，曾自然受孕，并且在体外受精检查过程中对促性腺激素刺激方案有良好的激素和卵泡反应，但她出现了明显的反复妊娠丢失，同时伴有hCG水平长时间升高。最初，通过凝胶色谱法在血清中检测到一种高分子量的hCG复合物。[I - 125]重组hCG与一份血清样本结合，随后与从同一样本中亲和纯化的IgG结合，表明存在一种hCG抗体。该抗血清具有特异性，对hCG的亲和力较低（Ka为1.4×10⁶升/摩尔），但结合容量较高（418纳摩尔/升）。与重组人卵泡刺激素、重组人黄体生成素、hCGα和hCGβ的交叉反应较低（
An anti-hCG autoantibody was found in a patient with a 9-yr history of secondary infertility. Although the patient had regular menstrual cycles, had conceived spontaneously, and had good hormonal and follicular responses to gonadotropic stimulation regimens during the in vitro fertilization work-up, she presented with apparent recurrent pregnancy loss associated with prolonged raised hCG levels. Initially the presence of a high mol wt hCG complex was demonstrated in the serum by gel chromatography. The binding of [I-125]recombinant hCG to a serum sample and subsequently to the affinity-purified IgG from the same sample revealed the presence of an hCG antibody. The antiserum was shown to be specific, with a low affinity (K-a, 1.4 x 10(6) liters/mol), but a high capacity (418 nmol/ liter), for hCG. Cross-reaction with recombinant human FSH, recombinant human LH, hCGalpha, and hCGbeta were low (