Mesenchymal stem cells: Isolation and therapeutics
Mesenchymal stem cells: Isolation and therapeutics
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DOI：
10.1089/scd.2004.13.436
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发表时间：
2004-08-01
影响因子：
4
通讯作者：
Mao, JJ
中科院分区：
文献类型：
--
作者：
Alhadlaq, A;Mao, JJ
间充质干细胞是所有结缔组织细胞的祖细胞。在多种脊椎动物的成年物种中，MSCs已从骨髓（BM）和其他组织中分离出来，在培养中扩增，并分化成几种组织形成细胞，如骨、软骨、脂肪、肌肉、肌腱、肝脏、肾脏、心脏甚至脑细胞。最近在将间充质干细胞应用于滑膜关节的人形关节髁的再生方面的实际进展是其功能性和多功能性的一个例子。本综述不仅概述了与MSCs的分离和治疗应用相关的几种方法，而且还介绍了分离的MSCs及其后代的表型和功能表征的几个例子。
Mesenchymal stem cells (MSCs) are progenitors of all connective tissue cells. In adults of multiple vertebrate species, MSCs have been isolated from bone marrow ( BM) and other tissues, expanded in culture, and differentiated into several tissue-forming cells such as bone, cartilage, fat, muscle, tendon, liver, kidney, heart, and even brain cells. Recent advances in the practical end of application of MSCs toward regeneration of a human-shaped articular condyle of the synovial joint is one example of their functionality and versatility. The present review not only outlines several approaches relevant to the isolation and therapeutic use of MSCs, but also presents several examples of phenotypic and functional characterization of isolated MSCs and their progeny.