Global Gene Expression Profiling and Alternative Splicing Events during the Chondrogenic Differentiation of Human Cartilage Endplate-Derived Stem Cells.
Global Gene Expression Profiling and Alternative Splicing Events during the Chondrogenic Differentiation of Human Cartilage Endplate-Derived Stem Cells.
复制标题
人软骨终板干细胞软骨分化过程中的整体基因表达谱和选择性剪接事件
DOI：
10.1155/2015/604972
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发表时间：
2015
影响因子：
--
通讯作者：
Zhou Y
中科院分区：
文献类型：
--
作者：
Shang J;Fan X;Shangguan L;Liu H;Zhou Y
下腰痛是一种非常普遍的疾病，椎间盘退行性疾病通常是下腰痛的主要原因。然而，DDDs的发病机制复杂，难以阐明。选择性剪接是一个复杂的调控过程，它极大地增加了真核生物的细胞复杂性和表型多样性。此外，本课题组还发现并鉴定了软骨终板源性干细胞。在本文中，我们继续调查基因表达谱和选择性剪接事件在软骨终板来源的干细胞软骨分化。我们采用Affyscore Human Transcriptome Array 2.0（HTA 2.0）比较对照和分化样品之间的转录和剪接变化。采用RT-PCR和定量PCR对芯片结果进行验证。还进行了GO和KEGG通路分析。对数据进行生物信息学分析后，共检测到1953个差异表达基因。在选择性剪接方面，使用剪接指数算法来选择选择性剪接基因。我们检测到4411个可变剪接基因。GO和KEGG通路分析还揭示了几个功能上涉及的生物过程和信号通路。据我们所知，这是第一个在全基因组范围内研究干细胞软骨分化的选择性剪接机制的研究。
Low back pain (LBP) is a very prevalent disease and degenerative disc diseases (DDDs) usually account for the LBP. However, the pathogenesis of DDDs is complicated and difficult to elucidate. Alternative splicing is a sophisticated regulatory process which greatly increases cellular complexity and phenotypic diversity of eukaryotic organisms. In addition, the cartilage endplate-derived stem cells have been discovered and identified by our research group. In this paper, we continue to investigate gene expression profiling and alternative splicing events during chondrogenic differentiation of cartilage endplate-derived stem cells. We adopted Affymetrix Human Transcriptome Array 2.0 (HTA 2.0) to compare the transcriptional and splicing changes between the control and differentiated samples. RT-PCR and quantitative PCR are used to validate the microarray results. The GO and KEGG pathway analysis was also performed. After bioinformatics analysis of the data, we detected 1953 differentially expressed genes. In terms of alternative splicing, the Splicing Index algorithm was used to select alternatively spliced genes. We detected 4411 alternatively spliced genes. GO and KEGG pathway analysis also revealed several functionally involved biological processes and signaling pathways. To our knowledge, this is the first study to investigate the alternative splicing mechanisms in chondrogenic differentiation of stem cells on a genome-wide scale.