Exploring lung physiology in health and disease with lung slices.
Exploring lung physiology in health and disease with lung slices.
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DOI：
10.1016/j.pupt.2011.05.001
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发表时间：
2011-10
影响因子：
3.2
通讯作者：
Sanderson MJ
中科院分区：
文献类型：
--
作者：
Sanderson MJ
发展治疗肺部疾病的方法需要了解肺的正常和疾病生理。虽然传统的实验方法只涉及器官或细胞生理学，但使用肺切片制剂提供了一种独特的方法来研究连接细胞和器官反应的综合生理学。活肺切片是健壮的，可以从包括人类在内的各种物种中制备，并且它们保留了肺的细胞和结构组织的许多方面。肺泡实质内可见肺内气道、小动脉和静脉的功能部分。与气道和血管相关的宏观收缩和松弛的动态变化很容易用传统的低倍显微镜观察到。与决定宏观反应的细胞事件相关的微观变化可以用共聚焦或双光子显微镜观察到。为了研究疾病过程，可以从疾病动物模型或暴露于疾病诱发条件的动物中制备肺切片。另外，肺切片本身也可以通过实验进行操作。由于能够观察细胞生理学的变化以及这些反应如何在器官水平上表现出来，肺切片已成为调查肺部疾病的标准工具。
The development of therapeutic approaches to treat lung disease requires an understanding of both the normal and disease physiology of the lung. Although traditional experimental approaches only address either organ or cellular physiology, the use of lung slice preparations provides a unique approach to investigate integrated physiology that links the cellular and organ responses. Living lung slices are robust and can be prepared from a variety of species, including humans, and they retain many aspects of the cellular and structural organization of the lung. Functional portions of intrapulmonary airways, arterioles and veins are present within the alveoli parenchyma. The dynamics of macroscopic changes of contraction and relaxation associated with the airways and vessels are readily observed with conventional low-magnification microscopy. The microscopic changes associated with cellular events, that determine the macroscopic responses, can be observed with confocal or two-photon microscopy. To investigate disease processes, lung slices can either be prepared from animal models of disease or animals exposed to disease invoking conditions. Alternatively, the lung slices themselves can be experimentally manipulated. Because of the ability to observe changes in cell physiology and how these responses manifest themselves at the level of the organ, lung slices have become a standard tool for the investigation of lung disease.
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影响因子：
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作者：
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影响因子：
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