Inactivation of Bacteriophages via photosensitization of fullerol nanoparticles
Inactivation of Bacteriophages via photosensitization of fullerol nanoparticles
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DOI：
10.1021/es0708215
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发表时间：
2007-09-15
影响因子：
11.4
通讯作者：
Wiesner, Mark R.
中科院分区：
文献类型：
--
作者：
Badireddy, Appala Raju;Hotze, Ernest M.;Wiesner, Mark R.
两个活性氧物种的生产，通过紫外线光敏化的多羟基富勒烯（富勒醇），以提高病毒灭活率。通过电子顺磁共振和硝基蓝四氮唑还原两种独特的方法证实了富勒醇在紫外光存在下产生单线态氧和超氧化物。这些发现建立在富勒烯光敏化和富勒烯对微型动物影响领域的先前结果的基础上。结果显示，与单独的UVA照射相比，由于单线态氧的存在，一级MS2噬菌体灭活率几乎翻了一番，并且由于单线态氧和超氧化物的存在，一级MS2噬菌体灭活率增加了125%。当溶液中存在富勒醇和NADH时，病毒的暗灭活发生的速率与不含纳米颗粒的UVA照射产生的速率几乎相同。这些结果表明，富勒烯有可能影响自然和工程系统中的病毒种群，从地表沃茨到水和废水处理的消毒技术。
The production of two reactive oxygen species through UV photosensitization of polyhydroxylated fullerene (fullerol) is shown to enhance viral inactivation rates. The production of both singlet oxygen and superoxide by fullerol in the presence of UV light is confirmed via two unique methods: electron paramagnetic resonance and reduction of nitro blue tetrazolium. These findings build on previous results both in the area of fullerene photosensitization and in the area of fullerene impact on microfauna. Results showed that the first-order MS2 bacteriophage inactivation rate nearly doubled due to the presence of singlet oxygen and increased by 125% due to singlet oxygen and superoxide as compared to UVA illumination alone. When fullerol and NADH are present in solution, dark inactivation of viruses occurs at nearly the same rate as that produced by UVA illumination without nanoparticles. These results suggest a potential for fullerenes to impact virus populations in both natural and engineered systems ranging from surface waters to disinfection technologies for water and wastewater treatment.