THE ISOLATION OF PEPTIDES BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY USING PREDICTED ELUTION POSITIONS
THE ISOLATION OF PEPTIDES BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY USING PREDICTED ELUTION POSITIONS
复制标题
DOI：
10.1016/0003-2697(82)90238-x
复制
发表时间：
1982-01-01
影响因子：
2.9
通讯作者：
SOLOMON, S
中科院分区：
文献类型：
--
作者：
BROWNE, CA;BENNETT, HPJ;SOLOMON, S
本文介绍了肽反相高效液相色谱预测保留系数的推导和使用。通过两个例子说明了预测洗脱位置在肽分离中的应用，其中根据 cDNA 序列数据推测其存在的肽已被成功分离。强大的色谱技术与根据肽的组成预测肽洗脱位置的能力相结合，为从生物组织中分离肽提供了一种非常有效的方法。
This paper describes the derivation and use of predictive retention coefficients for the reversed-phase high-performance liquid chromatography of peptides. The use of predicted elution positions in the isolation of peptides is illustrated by two examples where peptides, whose existence was postulated from cDNA sequence data, have been successfully isolated. The combination of the powerful chromatographic technology and the ability to predict the elution positions of peptides based on their composition provides a very potent method for the isolation of peptides from biological tissues.