dCtBP mediates transcriptional repression by Knirps, Kruppel and Snail in the Drosophila embryo
dCtBP mediates transcriptional repression by Knirps, Kruppel and Snail in the Drosophila embryo
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DOI：
10.1093/emboj/17.23.7009
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发表时间：
1998-12-01
期刊：
影响因子：
11.4
通讯作者：
Levine, M
中科院分区：
文献类型：
--
作者：
Nibu, Y;Zhang, HL;Levine, M
果蝇的前细胞胚胎包含10个特征良好的序列特异性转录抑制因子，它们代表了广泛的DNA结合蛋白。先前的研究表明，其中两个抑制子，毛发和背部，招募了一种共同的辅助抑制蛋白Groucho，这里我们提供的证据表明，三种不同的抑制子，Knirps，Kruppel和Snail，招募了另一种不同的辅助抑制物，dCtBP突变胚胎含有降低水平的母体dCtBP产物，同时表现出节段性和背腹图案缺陷，这可以归因于Kruppel，Knirps和Snail活性的丧失。相反，在dCtBP突变胚胎中，背部和毛发抑制子至少保留了一些活性。DCtBP通过与Kruppel、Knirps和Snail相关的序列Moth PXDLSXK/H与Kruppel、Knirps和Snail相互作用，该序列对转基因胚胎中这些蛋白的抑制活性是必不可少的。我们认为dCtBP代表了发育过程中转录抑制的一种主要形式，Groucho和dCtBP共抑制物介导了不同的抑制途径。
The pre-cellular Drosophila embryo contains 10 well characterized sequence-specific transcriptional repressors, which represent a broad spectrum of DNA-binding proteins. Previous studies have shown that two of the repressors, Hairy and Dorsal, recruit a common co-repressor protein, Groucho, Here we present evidence that three different repressors, Knirps, Kruppel and Snail, recruit a different co-repressor, dCtBP Mutant embryos containing diminished levels of maternal dCtBP products exhibit both segmentation and dorsoventral patterning defects, which can be attributed to loss of Kruppel, Knirps and Snail activity. Tn contrast, the Dorsal and Hairy repressors retain at least some activity in dCtBP mutant embryos. dCtBP interacts with Kruppel, Knirps and Snail through a related sequence moth, PXDLSXK/H, This moth is essential for the repression activity of these proteins in transgenic embryos. We propose that dCtBP represents a major form of transcriptional repression in development, and that the Groucho and dCtBP co-repressors mediate separate pathways of repression.