Persistence of novel human parvovirus PARV4 in liver tissue of adults
Persistence of novel human parvovirus PARV4 in liver tissue of adults
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DOI：
10.1002/jmv.21069
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发表时间：
2008-02-01
影响因子：
12.7
通讯作者：
Eis-Huebinger, Anna M.
中科院分区：
文献类型：
--
作者：
Schneider, Beate;Fryer, Jacqueline F.;Eis-Huebinger, Anna M.
人类细小病毒4型（PARV 4）是最近发现的一种病毒，其生物学、流行病学和致病潜力尚未确定。最近，据报道，PARV 4 DNA持续存在于一些HIV感染个体的组织中，而在未感染HIV的受试者的组织中未检测到PARV 4 DNA。在本研究中，分析了来自87名个体的肝组织中PARV 4 DNA的存在，这些个体中除一名个体外均未感染HIV。总体而言，在13份标本（15%）中检测到PARV 4 DNA。在检查的其他组织中，在四个配对的骨髓标本中的一个中检测到PARV 4基因型2（也称为PARV 5）DNA。组织病毒载量不超过100拷贝/μ g基因组DNA。此外，来自这些个体中的40个的血清样本对PARV 4 DNA检测均为阴性。在本研究分析的受试者中，PARV 4基因型2似乎是遗传性的。比PARV 4基因型1更异质。结果表明，PARV 4 DNA可以在肝脏中检测到，PARV 4的感染并不局限于HIV感染者。先前的研究显示PARV 4存在于血浆中，表明在PARV 4感染期间，发生病毒血症阶段，允许全身扩散到各种组织。
Human parvovirus 4 (PARV4) is a recently identified virus whose biology, epidemiology and pathogenic potential have yet to be determined. Recently, it was reported that PARV4 DNA persists in tissues of some HIV-infected individuals, whilst PARV4 DNA was not detected in tissues of subjects not infected with HIV. In the present study, liver tissue from 87 individuals, none of who were infected with HIV, with the exception of a single subject, was analyzed for the presence of PARV4 DNA. Overall, PARV4 DNA was detected in 13 specimens (15%). In other tissues examined, PARV4 genotype 2 (also termed PARV5) DNA was detected in one of four paired bone marrow specimens. Tissue viral loads did not exceed 100 copies per mu g of genomic DNA. In addition, serum samples from 40 of these individuals all tested negative for PARV4 DNA. In the subjects analyzed in this study, PARV4 genotype 2 appeared to be genetically. more heterogeneous than PARV4 genotype 1. The results show that PARV4 DNA can be detected in liver, and that infection with PARV4 is not restricted to HIV-infected individuals. Previous studies showing the presence of PARV4 in plasma, suggest that during infection with PARV4, a viraemic stage occurs, allowing systemic spread to a variety of tissues.