ced-10 Rac and mig-2 function redundantly and act with unc-73 trio to control the orientation of vulval cell divisions and migrations in Caenorhahditis elegans
ced-10 Rac and mig-2 function redundantly and act with unc-73 trio to control the orientation of vulval cell divisions and migrations in Caenorhahditis elegans
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DOI：
10.1006/dbio.2001.0513
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发表时间：
2002-01-15
影响因子：
2.7
通讯作者：
Sundaram, MV
中科院分区：
文献类型：
--
作者：
Kishore, RS;Sundaram, MV
外阴发育线虫秀丽隐杆线虫可分为一个命运规范阶段控制的部分让-60 Ras，和命运执行阶段涉及定型模式的细胞分裂和迁移控制的部分lin-17卷曲。由于小的GTd-Rac已被牵连作为Ras和Frizzled的下游靶点，并影响细胞骨架动力学，我们研究了Rac信号传导在外阴发育的每个阶段中的作用。我们发现，Rac基因ced-10和Rac相关基因mig-2是冗余需要某些外阴细胞分裂的正确方向，这表明在纺锤体定位的作用。Red-10 Rae和mig-2也是外阴细胞迁移所必需的，并且在外阴命运特化中起次要作用。mig-2的组成型活性和显性阴性突变体形式引起外阴缺陷，这与在red-10中观察到的非常相似;mig-2双重功能丧失突变体，表明它们干扰red-10 Rae和mig-2的功能。unc-73（一种Trio样鸟嘌呤核苷酸交换因子）的突变导致了类似的外阴缺陷，这表明nc-73是CED-10和nc-2的交换因子。我们讨论了细胞缺陷之间的相似性和差异，在Rae突变体和let-60 Ras或lin-17卷曲突变体。(C)2001年，爱思唯尔科学。
Vulval development in the nematode Caenorhabiditis elegans can be divided into a fate specification phase controlled in part by let-60 Ras, and a fate execution phase involving stereotypical patterns of cell division and migration controlled in part by lin-17 Frizzled. Since the small GTPase Rac has been implicated as a downstream target of both Ras and Frizzled and influences cytoskeletal dynamics, we investigated the role of Rac signaling during each phase of vulval development. We show that the Rac gene ced-10 and the Rac-related gene mig-2 are redundantly required for the proper orientation of certain vulval cell divisions, suggesting a role in spindle positioning. red-10 Rae and mig-2 are also redundantly required for vulval cell migrations and play a minor role in vulval fate specification. Constitutively active and dominant-negative mutant forms of mig-2 cause vulval defects that are very similar to those seen in red-10;mig-2 double loss-of-function mutants, indicating that they interfere with the functions of both red-10 Rae and mig-2. Mutations in unc-73 (a Trio-like guanine nucleotide exchange factor) cause similar vulval defects, suggesting that UNC-73 is an exchange factor for both CED-10 and MIG-2. We discuss the similarities and differences between the cellular defects seen in Rae mutants and let-60 Ras or lin-17 Frizzled mutants. (C) 2001 Elsevier Science.