DsbB is required for the pathogenesis process of Xanthomonas campestris pv. campestris
DsbB is required for the pathogenesis process of Xanthomonas campestris pv. campestris
复制标题
DsbB 是野油菜黄单胞菌 pv 的发病过程所必需的。
DOI：
10.1094/mpmi-21-8-1036
复制
发表时间：
2008-08-01
影响因子：
3.5
通讯作者：
Tang, Ji-Liang
中科院分区：
文献类型：
--
作者：
Jiang, Bo-Le;Liu, Jiao;Tang, Ji-Liang
DsbA/DsbB氧化途径是革兰氏阴性菌外质中催化蛋白质形成二硫键的两条途径之一。已经证明，DsbA是多种动物细菌性病原体的多种毒力因子所必需的。在这篇文章中，我们提出了遗传证据，表明开放阅读框XC_3314编码DsbB蛋白，该蛋白参与了油菜黄单胞菌外质中二硫键的形成。十字花科植物黑腐病的病原。油菜葡萄球菌的dsbB突变体。Campestris在植物中表现出毒力、超敏反应、细胞运动和细菌生长的衰减。此外，dsbB基因的突变导致II型和III型分泌系统以及鞭毛组装失效。这些发现表明，DsbB在葡萄球菌的发病过程中是必需的。定。
The DsbA/DsbB oxidation pathway is one of the two pathways that catalyze disulfide bond formation of proteins in the periplasm of gram-negative bacteria. It has been demonstrated that DsbA is essential for multiple virulence factors of several animal bacterial pathogens. In this article, we present genetic evidence to show that the open reading frame XC_3314 encodes a DsbB protein that is involved in disulfide bond formation in periplasm of Xanthomonas campestris pv. campestris, the causative agent of crucifer black rot disease. The dsbB mutant of X. campestris pv. campestris exhibited attenuation in virulence, hypersensitive response, cell motility, and bacterial growth in planta. Furthermore, mutation in the dsbB gene resulted in ineffective type II and type III secretion systems as well as flagellar assembly. These findings reveal that DsbB is required for the pathogenesis process of X. campestris pv. campestris.