Generation of a permanent cell line that supports efficient growth of Marek's disease virus (MDV) by constitutive expression of MDV glycoprotein E
Generation of a permanent cell line that supports efficient growth of Marek's disease virus (MDV) by constitutive expression of MDV glycoprotein E
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DOI：
10.1099/0022-1317-83-8-1987
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发表时间：
2002-08-01
影响因子：
3.8
通讯作者：
Osterrieder, N
中科院分区：
文献类型：
--
作者：
Schumacher, D;Tischer, BK;Osterrieder, N
从鹌鹑肌肉细胞系QM 7中获得了组成型表达马立克氏病病毒I型（MDV-1）糖蛋白E（gE）基因的重组细胞系（SOgE）。SOgE细胞系支持强毒（RB-1B）和疫苗（CV 1988，584 Ap80 C）MDV-1毒株的生长，其水平与原代鸡胚细胞（CEC）相当。SOgE细胞系用于生产针对马立克氏病的疫苗。在1日龄时用10（3）p.f.u. CV1988在CEC或SOgE细胞上产生。在第12天用高毒力意大利MDV-1毒株EU 1进行攻毒感染。然而，分别用SOgE或QM7细胞单独免疫的7/7或6/6只动物发生马立克氏病，来自两个CV1988免疫组的仅1/8只动物表现出疾病体征，表明SOgE细胞是用于MDV-1疫苗生产的有价值的永久细胞培养系统。
A recombinant cell line (SOgE) was established, which was derived from the permanent quail muscle cell line QM7 and constitutively expressed the glycoprotein E (gE) gene of Marek's disease virus serotype I (MDV-1). The SOgE cell line supported growth of virulent (RB-1B) and vaccine (CV1988, 584Ap80C) MDV-1 strains at a level comparable with that of primary chicken embryo cells (CEC). The SOgE cell line was used to produce a vaccine against Marek's disease. Chickens were immunized at I day old with 10(3) p.f.u. CV1988 produced on either CEC or SOgE cells. Challenge infection was performed at day 12 with hypervirulent Italian MDV-1 strain EU1. Whereas 7/7 or 6/6 animals, respectively, immunized with SOgE or QM7 cells alone developed Marek's disease, only 1/8 animals from both CV1988-immunized groups exhibited signs of disease, suggesting that SOgE cells are a valuable permanent cell culture system for MDV-1 vaccine production.