CALIBRATION OF [C-14] PLASTIC STANDARDS FOR QUANTITATIVE AUTORADIOGRAPHY OF [I-125]LABELED LIGANDS WITH AMERSHAM HYPERFILM BETA-MAX
CALIBRATION OF [C-14] PLASTIC STANDARDS FOR QUANTITATIVE AUTORADIOGRAPHY OF [I-125]LABELED LIGANDS WITH AMERSHAM HYPERFILM BETA-MAX
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DOI：
10.1016/0304-3940(89)90350-9
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发表时间：
1989-09-25
影响因子：
2.5
通讯作者：
WIMPY, TH
中科院分区：
文献类型：
--
作者：
BASKIN, DG;WIMPY, TH
Hyperfilm β的密度响应-将[14 C]塑料标准品的最大（HFBM）校准至[125 I]的组织等效浓度。将具有标准浓度[125 C]的塑料切片与HFBM贴壁1、3、5和9天，用[125 I]和[14 C]胰岛素标记肝脏和肌肉切片（20 μ m厚）。相对光密度（ROD = log 10 [1]/灰度×将[125 I]和[14 C]塑料标准品产生的[256-1]转化为等效组织[125 I]浓度（dpm/mm 2）。HFBM对[125 I]和[14 C]塑料标准品的反应相似（P < 0.001）。组织[125 I]（dpm/mm 2）对塑料[14 C]（μ Ci/g）浓度的标准曲线符合二阶多项式（r2 = 0.995-0.999）。结果表明，[14 C]塑料标准品用于HFBM放射自显影法测量组织切片中的[125 I]放射性是有效的。
The densitometric response of Hyperfilm .beta.-max (HFBM) to [14C] plastic standards was calibrated to tissue-equivalent concentrations of [125I]. Plastic sections with standard concentration of [125C] were apposed to HFBM for 1, 3, 5, and 9 days, with liver and muscle slices (20 .mu.m thick) labeled with [125I] and [14C] insulin. The relative optical densities (ROD = log10 [1/gray level .times. 256-1] produced by [125I]- and [14C] plastic standards were converted to equivalent tissue [125I] concentrations (dpm/mm2). The response of HFBM to the [125I]- and [14C] plastic standards was similar (P < 0.001). Standard curves of tissue [125I] (dpm/mm2) vs plastic [14C] (.mu.Ci/g) concentrations fit second order polynomials (r2 = 0.995-0.999). The results show that [14C] plastic standards are valid for measuring [125I] radioactivity in tissue slices by autoradiography with HFBM.