Fourier Transform Infrared Spectroscopic Imaging-Derived Collagen Content and Maturity Correlates with Stress in the Aortic Wall of Abdominal Aortic Aneurysm Patients.
Fourier Transform Infrared Spectroscopic Imaging-Derived Collagen Content and Maturity Correlates with Stress in the Aortic Wall of Abdominal Aortic Aneurysm Patients.
复制标题
傅里叶变换红外光谱成像衍生的胶原蛋白含量和成熟度与腹主动脉瘤患者主动脉壁的应力相关。
DOI：
10.1007/s13239-016-0289-3
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发表时间：
2017
影响因子：
1.8
通讯作者：
Pleshko,Nancy
中科院分区：
文献类型：
--
作者：
Cheheltani,Rabee;Pichamuthu,JosephE;Rao,Jayashree;Weinbaum,JustinS;Kiani,MohammadF;Vorp,DavidA;Pleshko,Nancy
腹主动脉瘤（AAA）是一种主动脉退行性疾病，其特征是主动脉壁及其主要分子成分的结构完整性严重破坏。从疾病的早期阶段，主动脉中的弹性蛋白变得高度降解，并被胶原蛋白取代。胶原蛋白含量、质量和成熟度对破裂可能性的贡献仍然存在问题。在这里，使用我们最近开发的傅里叶变换红外成像光谱（FT-IRIS）方法，我们量化的胶原蛋白含量和成熟度在AAA组织的壁对标本具有不同的壁应力。使用12名患者的AAA的CT扫描来创建有限元模型，以估计组织的不同区域中的应力。每例患者均接受了AAA的择期修复，切除后通过组织学和FT-IRIS评价了近端或远端具有不同壁应力的解剖区域的两段AAA组织。对于每例患者，胶原蛋白含量通常在具有较低壁应力的组织位置更高，这对应于更远端的解剖区域。高应力区的壁应力/胶原比大于低应力区（1.01 ± 1.09 vs. 0.55 ± 0.084，p = 0.02）。较高应力区域也对应于管腔内血栓厚度减少的位置。随着胶原含量的增加，胶原成熟度呈下降趋势（p= 0.068，R = 0.38）。总之，这些结果表明，AAA患者中较不成熟的胶原蛋白含量的增加不能有效补偿主动脉壁中弹性蛋白的损失，并导致耐受壁应力的能力降低。
Abdominal aortic aneurysm (AAA) is a degenerative disease of the aorta characterized by severe disruption of the structural integrity of the aortic wall and its major molecular constituents. From the early stages of disease, elastin in the aorta becomes highly degraded and is replaced by collagen. Questions persist as to the contribution of collagen content, quality and maturity to the potential for rupture. Here, using our recently developed Fourier transform infrared imaging spectroscopy (FT-IRIS) method, we quantified collagen content and maturity in the wall of AAA tissues in pairs of specimens with different wall stresses. CT scans of AAAs from 12 patients were used to create finite element models to estimate stress in different regions of tissue. Each patient underwent elective repair of the AAA, and two segments of the AAA tissues from anatomic regions more proximal or distal with different wall stresses were evaluated by histology and FT-IRIS after excision. For each patient, collagen content was generally greater in the tissue location with lower wall stress, which corresponded to the more distal anatomic regions. The wall stress/collagen ratio was greater in the higher stress region compared to the lower stress region (1.01 ± 1.09 vs. 0.55 ± 0.084,p= 0.02). The higher stress region also corresponded to the location with reduced intraluminal thrombus thickness. Further, collagen maturity tended to decrease with increased collagen content (p= 0.068,R= 0.38). Together, these results suggest that an increase in less mature collagen content in AAA patients does not effectively compensate for the loss of elastin in the aortic wall, and results in a reduced capability to endure wall stresses.